Cytotoxic effect of silica nanoparticle on some tumor cell lines
Joint Authors
Yasin, Nahi Yusuf
Salih, Khalid Mahdi
Asad, Shadha Salah
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 26, Issue 6 (30 Jun. 2018), pp.206-213, 8 p.
Publisher
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Objective: Silica nanoparticles have been discovered to exert cytotoxicity in normal and cancerous human cells.
Therefore, this study is aimed to evaluate the cytotoxic effect of silica nanoparticle on some tumor cell lines.
Materials and Methods: Six concentrations (100,200,400,600,800, and1000 μg/ml) of silicon oxide SiO2nanoparticles (SONPs)with particle size 20-30 nm were prepared and their cytotoxic effect was tested on Hela, RD and ANM3 cancer cell lines as well as REF normal cell line as a control for 24 and 48 hr by using MTT assay to estimate the optical density (OD) of cell growth at wave length 500nm.
Results: All tumor cell lines (RD, HeLa, and AMN3) demonstrated significant inhibition in the cell growth index (GI%) after 24 and 48 hr exposure to all concentrations of SONPs, however the GI% of Ref cell showed non-significant change after both incubation periods of treatment.
On the other hand, only RD cell line showed significant negative correlation (r=- 0.8178, P=0.047) between GI% and the concentration of SONPs after 24 hr exposure, while those HeLa, AMN3,and REF cell lines showed non-significant correlation whether after 24 hr or 48 hr oftreatment.
Conclusion: The SONP per se at diameter 20-30 nm revealed significant cytotoxic effect based on MTT assay indifferenttumor cell lines, and the magnitude oftheir cytotoxic effect is dose-independent in the majority of cell lines which indicated that they are acting in cell type-dependent manner.
Therefore, pathways by which SONPs induced their cytotoxic effect in tumor cells need further investigation.-
American Psychological Association (APA)
Asad, Shadha Salah& Salih, Khalid Mahdi& Yasin, Nahi Yusuf. 2018. Cytotoxic effect of silica nanoparticle on some tumor cell lines. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 26, no. 6, pp.206-213.
https://search.emarefa.net/detail/BIM-1120578
Modern Language Association (MLA)
Asad, Shadha Salah…[et al.]. Cytotoxic effect of silica nanoparticle on some tumor cell lines. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 26, no. 6 (2018), pp.206-213.
https://search.emarefa.net/detail/BIM-1120578
American Medical Association (AMA)
Asad, Shadha Salah& Salih, Khalid Mahdi& Yasin, Nahi Yusuf. Cytotoxic effect of silica nanoparticle on some tumor cell lines. Journal of Babylon University : Journal of Applied and Pure Sciences. 2018. Vol. 26, no. 6, pp.206-213.
https://search.emarefa.net/detail/BIM-1120578
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 211-213
Record ID
BIM-1120578