Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue
Joint Authors
Barbaro, Elena
Zangrando, Roberta
Barbante, Carlo
Gambaro, A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Domoic acid (DA), a neurotoxic amino acid produced by diatoms, is the main cause of amnesic shellfish poisoning (ASP).
In this work, we propose a very simple and fast analytical method to determine DA in mussel tissue.
The method consists of two consecutive extractions and requires no purification steps, due to a reduction of the extraction of the interfering species and the application of very sensitive and selective HILIC-MS/MS method.
The procedural method was validated through the estimation of trueness, extract yield, precision, detection, and quantification limits of analytical method.
The sample preparation was also evaluated through qualitative and quantitative evaluations of the matrix effect.
These evaluations were conducted both on the DA-free matrix spiked with known DA concentration and on the reference certified material (RCM).
We developed a very selective LC-MS/MS method with a very low value of method detection limit (9 ng g−1) without cleanup steps.
American Psychological Association (APA)
Barbaro, Elena& Zangrando, Roberta& Barbante, Carlo& Gambaro, A.. 2016. Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue. The Scientific World Journal،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1120621
Modern Language Association (MLA)
Barbaro, Elena…[et al.]. Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue. The Scientific World Journal No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1120621
American Medical Association (AMA)
Barbaro, Elena& Zangrando, Roberta& Barbante, Carlo& Gambaro, A.. Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue. The Scientific World Journal. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1120621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1120621