The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media
Joint Authors
Vetter, Anna M.
Helmecke, Julia
Schomburg, Dietmar
Neumann-Schaal, Meina
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Microorganisms are well adapted to their habitat but are partially sensitive to toxic metabolites or abiotic compounds secreted by other organisms or chemically formed under the respective environmental conditions.
Thermoacidophiles are challenged by pyroglutamate, a lactam that is spontaneously formed by cyclization of glutamate under aerobic thermoacidophilic conditions.
It is known that growth of the thermoacidophilic crenarchaeon Saccharolobus solfataricus (formerly Sulfolobus solfataricus) is completely inhibited by pyroglutamate.
In the present study, we investigated the effect of pyroglutamate on the growth of S.
solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius.
In contrast to S.
solfataricus, S.
acidocaldarius was successfully cultivated with pyroglutamate as a sole carbon source.
Bioinformatical analyses showed that both members of the Sulfolobaceae have at least one candidate for a 5-oxoprolinase, which catalyses the ATP-dependent conversion of pyroglutamate to glutamate.
In S.
solfataricus, we observed the intracellular accumulation of pyroglutamate and crude cell extract assays showed a less effective degradation of pyroglutamate.
Apparently, S.
acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to S.
solfataricus.
Concludingly, S.
acidocaldarius exhibits a more efficient utilization of pyroglutamate and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures.
American Psychological Association (APA)
Vetter, Anna M.& Helmecke, Julia& Schomburg, Dietmar& Neumann-Schaal, Meina. 2019. The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media. Archaea،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1122240
Modern Language Association (MLA)
Vetter, Anna M.…[et al.]. The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media. Archaea No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1122240
American Medical Association (AMA)
Vetter, Anna M.& Helmecke, Julia& Schomburg, Dietmar& Neumann-Schaal, Meina. The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media. Archaea. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1122240
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1122240