Optimization of an In Vitro TranscriptionTranslation System Based on Sulfolobus solfataricus Cell Lysate

Joint Authors

Lo Gullo, Giada
Mattossovich, Rosanna
Perugino, Giuseppe
La Teana, Anna
Benelli, Dario
Londei, Paola

Source

Archaea

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-02-11

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

A system is described which permits the efficient synthesis of proteins in vitro at high temperature.

It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for translation of pretranscribed mRNAs, and now adapted to perform coupled transcription and translation.

The essential element in this expression system is a strong promoter derived from the S.

solfataricus 16S/23S rRNA-encoding gene, from which specific mRNAs may be transcribed with high efficiency.

The synthesis of two different proteins is reported, including the S.

solfataricus DNA-alkylguanine-DNA-alkyl-transferase protein (SsOGT), which is shown to be successfully labeled with appropriate fluorescent substrates and visualized in cell extracts.

The simplicity of the experimental procedure and specific activity of the proteins offer a number of possibilities for the study of structure-function relationships of proteins.

American Psychological Association (APA)

Lo Gullo, Giada& Mattossovich, Rosanna& Perugino, Giuseppe& La Teana, Anna& Londei, Paola& Benelli, Dario. 2019. Optimization of an In Vitro TranscriptionTranslation System Based on Sulfolobus solfataricus Cell Lysate. Archaea،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1122252

Modern Language Association (MLA)

Lo Gullo, Giada…[et al.]. Optimization of an In Vitro TranscriptionTranslation System Based on Sulfolobus solfataricus Cell Lysate. Archaea No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1122252

American Medical Association (AMA)

Lo Gullo, Giada& Mattossovich, Rosanna& Perugino, Giuseppe& La Teana, Anna& Londei, Paola& Benelli, Dario. Optimization of an In Vitro TranscriptionTranslation System Based on Sulfolobus solfataricus Cell Lysate. Archaea. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1122252

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1122252