Molecular Characterization of African Swine Fever Viruses from Outbreaks in Peri-Urban Kampala, Uganda
Joint Authors
Nkamwesiga, Joseph
Ndekezi, Christian
Ochwo, Sylvester
Norbert Mwiine, Frank
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
African swine fever (ASF) is an infectious transboundary disease of domestic pigs and wild swine and is currently the most serious constraint to piggery in Uganda.
The causative agent of ASF is a large double-stranded linear DNA virus with a complex structure.
There are twenty-four ASFV genotypes described to date; however, in Uganda, only genotypes IX and X have been previously described.
Inadequate ASF outbreak investigation has contributed to the delayed establishment of effective interventions to aid the control of ASF.
Continuous virus characterization enhances the understanding of ASF epidemiology in terms of viral genome variations, extent, severity, and the potential source of the viruses responsible for outbreaks.
We collected samples from pigs that had died of a hemorrhagic disease indicative of ASF.
DNA was extracted from all samples and screened with the OIE recommended diagnostic PCR for ASF.
Partial B646L (p72), full-length E183L (p54) genes, and CVR region of the P72 gene were amplified, purified, and sequenced.
Web-based BLAST and MEGA X software were used for sequence analysis.
ASF was confirmed in 10 of the 15 suspected pig samples.
Phylogenetic analysis confirmed circulation of genotype IX by both full-length E183 (p54) and partial B646L (p72) gene sequencing.
Intragenotypic resolution of the CVR region revealed major deletions in the virus genome, in some isolates of this study.
The marked reduction in the number of tetrameric tandem repeats in some isolates of this study could potentially play a role in influencing the virulence of this particular genotype IX in Uganda.
American Psychological Association (APA)
Norbert Mwiine, Frank& Nkamwesiga, Joseph& Ndekezi, Christian& Ochwo, Sylvester. 2019. Molecular Characterization of African Swine Fever Viruses from Outbreaks in Peri-Urban Kampala, Uganda. Advances in Virology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1122433
Modern Language Association (MLA)
Norbert Mwiine, Frank…[et al.]. Molecular Characterization of African Swine Fever Viruses from Outbreaks in Peri-Urban Kampala, Uganda. Advances in Virology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1122433
American Medical Association (AMA)
Norbert Mwiine, Frank& Nkamwesiga, Joseph& Ndekezi, Christian& Ochwo, Sylvester. Molecular Characterization of African Swine Fever Viruses from Outbreaks in Peri-Urban Kampala, Uganda. Advances in Virology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1122433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1122433