Hydrogenotrophic Methanogenesis and Autotrophic Growth of Methanosarcina thermophila
Joint Authors
Lackner, Nina
Hintersonnleitner, Anna
Wagner, Andreas Otto
Illmer, Paul
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Although Methanosarcinales are versatile concerning their methanogenic substrates, the ability of Methanosarcina thermophila to use carbon dioxide (CO2) for catabolic and anabolic metabolism was not proven until now.
Here, we show that M.
thermophila used CO2 to perform hydrogenotrophic methanogenesis in the presence as well as in the absence of methanol.
During incubation with hydrogen, the methanogen utilized the substrates methanol and CO2 consecutively, resulting in a biphasic methane production.
Growth exclusively from CO2 occurred slowly but reproducibly with concomitant production of biomass, verified by DNA quantification.
Besides verification through multiple transfers into fresh medium, the identity of the culture was confirmed by 16s RNA sequencing, and the incorporation of carbon atoms from 13CO2 into 13CH4 molecules was measured to validate the obtained data.
New insights into the physiology of M.
thermophila can serve as reference for genomic analyses to link genes with metabolic features in uncultured organisms.
American Psychological Association (APA)
Lackner, Nina& Hintersonnleitner, Anna& Wagner, Andreas Otto& Illmer, Paul. 2018. Hydrogenotrophic Methanogenesis and Autotrophic Growth of Methanosarcina thermophila. Archaea،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1122821
Modern Language Association (MLA)
Lackner, Nina…[et al.]. Hydrogenotrophic Methanogenesis and Autotrophic Growth of Methanosarcina thermophila. Archaea No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1122821
American Medical Association (AMA)
Lackner, Nina& Hintersonnleitner, Anna& Wagner, Andreas Otto& Illmer, Paul. Hydrogenotrophic Methanogenesis and Autotrophic Growth of Methanosarcina thermophila. Archaea. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1122821
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1122821