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Apoptotic and Early Innate Immune Responses to PB1-F2 Protein of Influenza A Viruses Belonging to Different Subtypes in Human Lung Epithelial A549 Cells
Joint Authors
Pasricha, Gunisha
Mukherjee, Sanjay
Chakrabarti, Alok K.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
PB1-F2 is a multifunctional protein and contributes to the pathogenicity of influenza A viruses.
PB1-F2 is known to have strain and cell specific functions.
In this study we have investigated the apoptotic and inflammatory responses of PB1-F2 protein from influenza viruses of diverse pathogenicities in A549 lung epithelial cells.
Overexpression of PB1-F2 resulted in apoptosis and heightened inflammatory response in A549 cells.
Comparison revealed that the response varied with each subtype.
PB1-F2 protein from highly pathogenic H5N1 virus induced least apoptosis but maximum inflammatory response.
Results indicated that apoptosis was mediated through death receptor ligands TNFα and TRAIL via Caspase 8 activation.
Significant induction of cytokines/chemokines CXCL10, CCL5, CCL2, IFNα, and IL-6 was noted in A549 cells transfected with PB1-F2 gene construct of 2008 West Bengal H5N1 virus (H5N1-WB).
On the contrary, PB1-F2 construct from 2007 highly pathogenic H5N1 isolate (H5N1-M) with truncated N-terminal region did not evoke as exuberant inflammatory response as the other H5N1-WB with full length PB1-F2, signifying the importance of N-terminal region of PB1-F2.
Sequence analysis revealed that PB1-F2 proteins derived from different influenza viruses varied at multiple amino acid positions.
The secondary structure prediction showed each of the PB1-F2 proteins had distinct helix-loop-helix structure.
Thus, our data substantiate the notion that the contribution of PB1-F2 to influenza pathogenicity is greatly strain specific and involves multiple host factors.
This data demonstrates that PB1-F2 protein of influenza A virus, when expressed independently is minimally apoptotic and strongly influences the early host response in A549 cells.
American Psychological Association (APA)
Pasricha, Gunisha& Mukherjee, Sanjay& Chakrabarti, Alok K.. 2018. Apoptotic and Early Innate Immune Responses to PB1-F2 Protein of Influenza A Viruses Belonging to Different Subtypes in Human Lung Epithelial A549 Cells. Advances in Virology،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1123058
Modern Language Association (MLA)
Pasricha, Gunisha…[et al.]. Apoptotic and Early Innate Immune Responses to PB1-F2 Protein of Influenza A Viruses Belonging to Different Subtypes in Human Lung Epithelial A549 Cells. Advances in Virology No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1123058
American Medical Association (AMA)
Pasricha, Gunisha& Mukherjee, Sanjay& Chakrabarti, Alok K.. Apoptotic and Early Innate Immune Responses to PB1-F2 Protein of Influenza A Viruses Belonging to Different Subtypes in Human Lung Epithelial A549 Cells. Advances in Virology. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1123058
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123058