![](/images/graphics-bg.png)
Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model
Joint Authors
Uchida, Kentaro
Matsuura, Terumasa
Saito, Wataru
Minehara, Hiroaki
Kawamura, Tadashi
Tazawa, Ryo
Takaso, Masashi
Inoue, Gen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study aimed to investigate whether a single injection of recombinant human bone morphogenetic protein-2-loaded artificial collagen-like peptide gel (rhBMP-2/ACG) accelerates consolidation at the bone defect site and bone union at the docking site in a mouse segmental bone transport (SBT) model.
A critical sized bone defect (2 mm) was created in the femur of mice and subsequently reconstructed using SBT with an external fixator.
Mice were divided into four treatment groups: Group CONT (immobile control), Group 0.2 (bone segments moved 0.2 mm/day for 10 days), Group 1.0 (bone segments moved 1.0 mm/day for 2 days), and Group 1.0/BMP-2 (rhBMP-2/ACG injected into the bone defect and segments moved 1.0 mm/day for 2 days).
Consolidation at the bone defect site and bone union at the docking site was evaluated radiologically and histologically across eight weeks.
Bone volume and bone mineral content were significantly higher in Group 0.2 than in Group 1.0.
Group 0.2 showed evidence of rebuilding of the medullary canal eight weeks after surgery at the bone defect site.
However, in Group 1.0, maturation of regenerative bone at the bone defect site was poor, with the central area between the proximal and distal bone composed mainly of masses of fibrous and adipose tissue.
Group 1.0/BMP-2 had higher bone volume and bone mineral content compared to Group 1.0, and all mice achieved bone union at the bone defect and docking sites.
Single injection of rhBMP-2/ACG combined with SBT may be effective for enhancing bone healing in large bone defects.
American Psychological Association (APA)
Tazawa, Ryo& Minehara, Hiroaki& Matsuura, Terumasa& Kawamura, Tadashi& Uchida, Kentaro& Inoue, Gen…[et al.]. 2019. Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1123098
Modern Language Association (MLA)
Tazawa, Ryo…[et al.]. Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1123098
American Medical Association (AMA)
Tazawa, Ryo& Minehara, Hiroaki& Matsuura, Terumasa& Kawamura, Tadashi& Uchida, Kentaro& Inoue, Gen…[et al.]. Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1123098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123098