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Computational Approach for Structural Feature Determination of Grapevine NHX Antiporters
Joint Authors
Aifa, Sami
Ayadi, Mariem
Ben Ayed, Rayda
Mzid, Rim
Hanana, Mohsen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Plant NHX antiporters are responsible for monovalent cation/H+ exchange across cellular membranes and play therefore a critical role for cellular pH regulation, Na+ and K+ homeostasis, and salt tolerance.
Six members of grapevine NHX family (VvNHX1-6) have been structurally characterized.
Phylogenetic analysis revealed their organization in two groups: VvNHX1-5 belonging to group I (vacuolar) and VvNHX6 belonging to group II (endosomal).
Conserved domain analysis of these VvNHXs indicates the presence of different kinds of domains.
Out of these, two domains function as monovalent cation-proton antiporters and one as the aspartate-alanine exchange; the remaining are not yet with defined function.
Overall, VvNHXs proteins are typically made of 11-13 putative transmembrane regions at their N-terminus which contain the consensus amiloride-binding domain in the 3rd TM domain and a cation-binding site in between the 5th and 6th TM domain, followed by a hydrophilic C-terminus that is the target of several and diverse regulatory posttranslational modifications.
Using a combination of primary structure analysis, secondary structure alignments, and the tertiary structural models, the VvNHXs revealed mainly 18 α helices although without β sheets.
Homology modeling of the 3D structure showed that VvNHX antiporters are similar to the bacterial sodium proton antiporters MjNhaP1 (Methanocaldococcus jannaschii) and PaNhaP (Pyrococcus abyssi).
American Psychological Association (APA)
Ayadi, Mariem& Ben Ayed, Rayda& Mzid, Rim& Aifa, Sami& Hanana, Mohsen. 2019. Computational Approach for Structural Feature Determination of Grapevine NHX Antiporters. BioMed Research International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1123118
Modern Language Association (MLA)
Ayadi, Mariem…[et al.]. Computational Approach for Structural Feature Determination of Grapevine NHX Antiporters. BioMed Research International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1123118
American Medical Association (AMA)
Ayadi, Mariem& Ben Ayed, Rayda& Mzid, Rim& Aifa, Sami& Hanana, Mohsen. Computational Approach for Structural Feature Determination of Grapevine NHX Antiporters. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1123118
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123118