Development of Clustered Resistance Gene Analogs-Based Markers of Resistance to Phytophthora capsici in Chili Pepper
Joint Authors
Kim, Nayoung
Kang, Won-Hee
Lee, Jundae
Yeom, Seon-In
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The soil-borne pathogen Phytophthora capsici causes severe destruction of Capsicum spp.
Resistance in Capsicum against P.
capsici is controlled by numerous minor quantitative trait loci (QTLs) and a consistent major QTL on chromosome 5.
Molecular markers on Capsicum chromosome 5 have been developed to identify the predominant genetic contributor to resistance but have achieved little success.
In this study, previously reported molecular markers were used to reanalyze the major QTL region on chromosome 5 (6.2 Mbp to 139.2 Mbp).
Candidate resistance gene analogs (RGAs) were identified in the extended major QTL region including 14 nucleotide binding site leucine-rich repeats, 3 receptor-like kinases, and 1 receptor-like protein.
Sequence comparison of the candidate RGAs was performed between two Capsicum germplasms that are resistant and susceptible, respectively, to P.
capsici.
11 novel RGA-based markers were developed through high-resolution melting analysis which were closely linked to the major QTL for P.
capsici resistance.
Among the markers, CaNB-5480 showed the highest cosegregation rate at 86.9% and can be applied to genotyping of the germplasms that were not amenable by previous markers.
With combination of three markers such as CaNB-5480, CaRP-5130 and CaNB-5330 increased genotyping accuracy for 61 Capsicum accessions.
These could be useful to facilitate high-throughput germplasm screening and further characterize resistance genes against P.
capsici in pepper.
American Psychological Association (APA)
Kim, Nayoung& Kang, Won-Hee& Lee, Jundae& Yeom, Seon-In. 2019. Development of Clustered Resistance Gene Analogs-Based Markers of Resistance to Phytophthora capsici in Chili Pepper. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123172
Modern Language Association (MLA)
Kim, Nayoung…[et al.]. Development of Clustered Resistance Gene Analogs-Based Markers of Resistance to Phytophthora capsici in Chili Pepper. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1123172
American Medical Association (AMA)
Kim, Nayoung& Kang, Won-Hee& Lee, Jundae& Yeom, Seon-In. Development of Clustered Resistance Gene Analogs-Based Markers of Resistance to Phytophthora capsici in Chili Pepper. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123172
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123172