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Structural Basis for pH-Dependent Oligomerization of Dihydropyrimidinase from Pseudomonas aeruginosa PAO1
Joint Authors
Huang, Cheng-Yang
Cheng, Jen-Hao
Huang, Chien-Chih
Huang, Yen-Hua
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Dihydropyrimidinase, a dimetalloenzyme containing a carboxylated lysine within the active site, is a member of the cyclic amidohydrolase family, which also includes allantoinase, dihydroorotase, hydantoinase, and imidase.
Unlike all known dihydropyrimidinases, which are tetrameric, pseudomonal dihydropyrimidinase forms a dimer at neutral pH.
In this paper, we report the crystal structure of P.
aeruginosa dihydropyrimidinase at pH 5.9 (PDB entry 5YKD).
The crystals of P.
aeruginosa dihydropyrimidinase belonged to space group C2221 with cell dimensions of a = 108.9, b = 155.7, and c = 235.6 Å.
The structure of P.
aeruginosa dihydropyrimidinase was solved at 2.17 Å resolution.
An asymmetric unit of the crystal contained four crystallographically independent P.
aeruginosa dihydropyrimidinase monomers.
Gel filtration chromatographic analysis of purified P.
aeruginosa dihydropyrimidinase revealed a mixture of dimers and tetramers at pH 5.9.
Thus, P.
aeruginosa dihydropyrimidinase can form a stable tetramer both in the crystalline state and in the solution.
Based on sequence analysis and structural comparison of the dimer-dimer interface between P.
aeruginosa dihydropyrimidinase and Thermus sp.
dihydropyrimidinase, different oligomerization mechanisms are proposed.
American Psychological Association (APA)
Cheng, Jen-Hao& Huang, Chien-Chih& Huang, Yen-Hua& Huang, Cheng-Yang. 2018. Structural Basis for pH-Dependent Oligomerization of Dihydropyrimidinase from Pseudomonas aeruginosa PAO1. Bioinorganic Chemistry and Applications،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1123236
Modern Language Association (MLA)
Cheng, Jen-Hao…[et al.]. Structural Basis for pH-Dependent Oligomerization of Dihydropyrimidinase from Pseudomonas aeruginosa PAO1. Bioinorganic Chemistry and Applications No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1123236
American Medical Association (AMA)
Cheng, Jen-Hao& Huang, Chien-Chih& Huang, Yen-Hua& Huang, Cheng-Yang. Structural Basis for pH-Dependent Oligomerization of Dihydropyrimidinase from Pseudomonas aeruginosa PAO1. Bioinorganic Chemistry and Applications. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1123236
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123236