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Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos)
Joint Authors
Xu, Qi
Chen, Guohong
Gu, Tiantian
Wu, Ningzhao
Zhang, Yang
Huang, Yu
Du, Jinping
Lu, Lizhi
Wu, Xinsheng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
SERPINA1 is a member of serine protease inhibitors and is increasingly considered to be a regulator of innate immunity in human and animals.
However, the expression and function of SERPINA1 gene in immune defense against viral infection remain unknown in ducks.
The full-length du SERPINA1 cDNA sequence was obtained using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE).
It contained 1457 nucleotide, including 47-bp 5’ UTR, 135-bp 3’ UTR, and 1275-bp open reading frame (ORF), and encodes a 424-amino acid protein.
Then, the tissue expression profile of du SERPINA1 gene was determined.
Real-time quantitative polymerase chain reaction (real-time qPCR) analysis revealed that du SERPINA1 mRNA is ubiquitous in various tissues, but higher expression levels were observed in lung and liver tissues.
In addition, the expression pattern was investigated when the ducklings were challenged with duck hepatitis virus 1(DHV-1) and polyriboinosinic polyribocytidylic acid (poly I:C).
After DHV-1 injection or poly I:C treatment, du SERPINA1 mRNA was up-regulated in the liver and kidney tissues.
However, the peak time in two tissues was not consistent.
In kidney, the expression lever of SERPINA1 increased immediately after the treatment while in liver tissue it kept steady until 12 h post-infection.
Our results indicate that SERPINA1 has an active role in the antiviral response, and thus improve our understanding of the role of this protein.
American Psychological Association (APA)
Gu, Tiantian& Wu, Ningzhao& Zhang, Yang& Huang, Yu& Du, Jinping& Lu, Lizhi…[et al.]. 2019. Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos). BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1123272
Modern Language Association (MLA)
Gu, Tiantian…[et al.]. Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos). BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1123272
American Medical Association (AMA)
Gu, Tiantian& Wu, Ningzhao& Zhang, Yang& Huang, Yu& Du, Jinping& Lu, Lizhi…[et al.]. Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos). BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1123272
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123272