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Dihydroartemisinin Protects against Dextran Sulfate Sodium-Induced Colitis in Mice through Inhibiting the PI3KAKT and NF-κB Signaling Pathways
Joint Authors
Chen, Dongfeng
Li, Ning
Sun, Wenjing
Zhou, Xin
Gong, Hao
Chen, Yuqing
Xiang, Fei
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Ulcerative colitis is a common inflammatory bowel disease, and the activation of thePI3K/AKT and NF-κB signaling pathways plays a pivotal role in its pathogenesis.
Dihydroartemisinin (DHA) is a widely used antimalarial drug and has shown anticancer effect partially through inhibiting the activation of PI3K/AKT and NF-κB.
This study aimed to investigate the effect of dihydroartemisinin on ulcerative colitis and its mechanism.
Adult male C57 mice were subjected to 3.0% dextran sulfate sodium (DSS) for seven days; simultaneously, dihydroartemisinin or control saline was administered by oral gavage once a day.
In vitro, the intestinal epithelial cell-6 was treated with LPS for 24 hours with or without dihydroartemisinin combined with PI3K/Akt activator 740 Y-P or NF-κB activator phorbol myristate acetate.
Western blotting was used to test the activation of PI3K/AKT and NF-κB.
Dihydroartemisinin significantly ameliorated body weight loss, shortened colon length, and increased DAI in DSS-induced colitis.
Meanwhile, histological damage was improved and was accompanied by decreased expression and secretion of proinflammatory cytokines.
Moreover, DSS-induced elevation of phosphorylation of PI3K, AKT, IKKα, IκBα, and NF-κB (p65) was remarkably blunted by dihydroartemisinin both in vivo and in vitro, indicating an inhibitive property on the PI3K/AKT and NF-κB signaling pathways.
Furthermore, administration of 740 Y-P or PMA significantly blocked protective activity of dihydroartemisinin against colitis in vitro.
In conclusion, dihydroartemisinin can attenuate DSS-induced colitis, and its anticolitis effect might be mediated via the PI3K/AKT and NF-κB signaling pathways.
DHA might serve as a promising drug for patients with ulcerative colitis.
American Psychological Association (APA)
Li, Ning& Sun, Wenjing& Zhou, Xin& Gong, Hao& Chen, Yuqing& Chen, Dongfeng…[et al.]. 2019. Dihydroartemisinin Protects against Dextran Sulfate Sodium-Induced Colitis in Mice through Inhibiting the PI3KAKT and NF-κB Signaling Pathways. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123330
Modern Language Association (MLA)
Li, Ning…[et al.]. Dihydroartemisinin Protects against Dextran Sulfate Sodium-Induced Colitis in Mice through Inhibiting the PI3KAKT and NF-κB Signaling Pathways. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1123330
American Medical Association (AMA)
Li, Ning& Sun, Wenjing& Zhou, Xin& Gong, Hao& Chen, Yuqing& Chen, Dongfeng…[et al.]. Dihydroartemisinin Protects against Dextran Sulfate Sodium-Induced Colitis in Mice through Inhibiting the PI3KAKT and NF-κB Signaling Pathways. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123330
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123330