TFIIIB Subunit Bdp1 Participates in RNA Polymerase III Transcription in the Protozoan Parasite Leishmania major

Abstract EN

Leishmania major, a protozoan parasite that diverged early from the main eukaryotic lineage, exhibits unusual mechanisms of gene expression.

Little is known in this organism about the transcription factors involved in the synthesis of tRNA, 5S rRNA, and snRNAs, transcribed by RNA Polymerase III (Pol III).

Here we identify and characterize the TFIIIB subunit Bdp1 in L.

major (LmBdp1).

Bdp1 plays key roles in Pol III transcription initiation in other organisms, as it participates in Pol III recruitment and promoter opening.

In silico analysis showed that LmBdp1 contains the typical extended SANT domain as well as other Bdp1 conserved regions.

Nevertheless, LmBdp1 also displays distinctive features, including the presence of only one aromatic residue in the N-linker region.

We were not able to produce null mutants of LmBdp1 by homologous recombination, as the obtained double replacement cell line contained an extra copy of LmBdp1, indicating that LmBdp1 is essential for the viability of L.

major promastigotes.

Notably, the mutant cell line showed reduced levels of the LmBdp1 protein, and its growth was significantly decreased in relation to wild-type cells.

Nuclear run-on assays demonstrated that Pol III transcription was affected in the mutant cell line, and ChIP experiments showed that LmBdp1 binds to 5S rRNA, tRNA, and snRNA genes.

Thus, our results indicate that LmBdp1 is an essential protein required for Pol III transcription in L.

major.