Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature
Joint Authors
Zhao, Xu
Yu, Changxia
Zhao, Yan
Liu, Shunjie
Wang, Hong
Wang, Chenguang
Guo, Ligang
Chen, Mingjie
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The mechanism of autolysis of Volvariella volvacea (V.
volvacea) at low temperature has not been fully explained.
As mannitol is among the most important osmotic adjustment substances in fungal resistance, this study sampled mycelia of strains V23 and VH3 treated at 0°C for 0, 2, 4, 8, and 10 h to analyze changes in intracellular mannitol content by high-performance anion chromatography with pulsed amperometric detection (HAPEC-PAD).
Reverse transcription quantitative PCR (RT-qPCR) analysis was applied to assess differences in the transcript levels of genes associated with mannitol metabolism under low-temperature stress.
A mannitol solution was added to cultures of V.
volvacea fruiting bodies, and effects on the hypothermic resistance of these organs were explored by evaluating variations in sensory properties during cryogenic storage after harvest.
The results suggested that in the initial stage of low-temperature treatment, intracellular mannitol was largely catabolized as an energy storage material and the expression of genes encoding enzymes involved in synthetic reactions was inhibited.
However, low-temperature resistance was induced with further treatment, with activation of mannitol synthesis and inhibition of degradation; the cells accumulated mannitol, leading to osmoregulation.
No significant elongation of V.
volvacea fruiting bodies during storage at 4°C was observed, and these organs tended to shrink and collapse.
The sensory quality of mannitol-treated fruiting bodies was much better than that of control fruiting bodies.
Application of a mannitol solution at the cultivation stage of V.
volvacea somewhat improved the low-temperature resistance of the fruiting bodies, verifying the correlation between mannitol and resistance to this stress in V.
volvacea.
The results of this study lay a foundation for a deeper understanding of the autolysis mechanism of V.
volvacea, providing technical support for increasing the cryopreservation time of this species and extending the postharvest shelf life of its fruiting bodies.
In addition, the mechanism underlying the low-temperature tolerance of the VH3 strain should be further explained at the molecular level.
American Psychological Association (APA)
Zhao, Xu& Yu, Changxia& Zhao, Yan& Liu, Shunjie& Wang, Hong& Wang, Chenguang…[et al.]. 2019. Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123394
Modern Language Association (MLA)
Zhao, Xu…[et al.]. Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1123394
American Medical Association (AMA)
Zhao, Xu& Yu, Changxia& Zhao, Yan& Liu, Shunjie& Wang, Hong& Wang, Chenguang…[et al.]. Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1123394
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123394