ITRAQ-Based Quantitative Proteomics Reveals the Proteome Profiles of Primary Duck Embryo Fibroblast Cells Infected with Duck Tembusu Virus
Joint Authors
Hu, Feng
Li, Yufeng
Yu, Kexiang
Huang, Bing
Ma, Xiuli
Liu, Cunxia
Guo, Xiaozhen
Song, Minxun
Wu, Jiaqiang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-27
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Outbreaks of duck Tembusu virus (DTMUV) have caused substantial economic losses in the major duck-producing regions of China since 2010.
To improve our understanding of the host cellular responses to virus infection and the pathogenesis of DTMUV infection, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with multidimensional liquid chromatography-tandem mass spectrometry to detect the protein changes in duck embryo fibroblast cells (DEFs) infected and mock-infected with DTMUV.
In total, 434 cellular proteins were differentially expressed, among which 116, 76, and 339 proteins were differentially expressed in the DTMUV-infected DEFs at 12, 24, and 42 hours postinfection, respectively.
The Gene Ontology analysis indicated that the biological processes of the differentially expressed proteins were primarily related to cellular processes, metabolic processes, biological regulation, response to stimulus, and cellular organismal processes and that the molecular functions in which the differentially expressed proteins were mainly involved were binding and catalytic activity.
Some selected proteins that were found to be differentially expressed in DTMUV-infected DEFs were further confirmed by real-time PCR.
The results of this study provide valuable insight into DTMUV-host interactions.
This could lead to a better understanding of DTMUV infection mechanisms.
American Psychological Association (APA)
Hu, Feng& Li, Yufeng& Yu, Kexiang& Huang, Bing& Ma, Xiuli& Liu, Cunxia…[et al.]. 2019. ITRAQ-Based Quantitative Proteomics Reveals the Proteome Profiles of Primary Duck Embryo Fibroblast Cells Infected with Duck Tembusu Virus. BioMed Research International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1123420
Modern Language Association (MLA)
Hu, Feng…[et al.]. ITRAQ-Based Quantitative Proteomics Reveals the Proteome Profiles of Primary Duck Embryo Fibroblast Cells Infected with Duck Tembusu Virus. BioMed Research International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1123420
American Medical Association (AMA)
Hu, Feng& Li, Yufeng& Yu, Kexiang& Huang, Bing& Ma, Xiuli& Liu, Cunxia…[et al.]. ITRAQ-Based Quantitative Proteomics Reveals the Proteome Profiles of Primary Duck Embryo Fibroblast Cells Infected with Duck Tembusu Virus. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1123420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123420