Rapid and Simple Detection of Trichosporon asahii by Optimized Colony PCR
Joint Authors
Liao, Yong
Lu, Xuelian
Zhang, Dequan
Xia, Zhikuan
Peng, Zhuoying
Yang, Xin
Yang, Rongya
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Trichosporon asahii is the major pathogen causing invasive trichosporonosis.
Conventional methods of its detection are time-consuming or costly and often require complex DNA extraction and purification steps, which hinders rapid clinical diagnosis.
In this study, we evaluated colony PCR, which directly uses colonies or trace clinical samples as the template for amplification, for rapid detection of T.
asahii infection.
Four methods, namely, direct colony, freeze-thaw, glass beads, and enzymolysis, were compared to select the best DNA extraction strategy.
We subsequently designed and screened species-specific primers targeting the intergenic spacer 1 (IGS1) of the ribosomal DNA of T.
asahii and used them to detect mock infection clinical samples.
The species-specific colony PCR based on glass beads proved advantageous, with short procedure time (154.8 ± 0.6 min), good sensitivity (detection limit, 102 CFU/mL), and specificity for T.
asahii, indicating that this method can be used for the rapid and simple identification of clinical samples of T.
asahii infection.
American Psychological Association (APA)
Zhang, Dequan& Lu, Xuelian& Liao, Yong& Xia, Zhikuan& Peng, Zhuoying& Yang, Xin…[et al.]. 2019. Rapid and Simple Detection of Trichosporon asahii by Optimized Colony PCR. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123514
Modern Language Association (MLA)
Zhang, Dequan…[et al.]. Rapid and Simple Detection of Trichosporon asahii by Optimized Colony PCR. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1123514
American Medical Association (AMA)
Zhang, Dequan& Lu, Xuelian& Liao, Yong& Xia, Zhikuan& Peng, Zhuoying& Yang, Xin…[et al.]. Rapid and Simple Detection of Trichosporon asahii by Optimized Colony PCR. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1123514
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123514