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The Effect of Tanreqing Injection on the Pharmacokinetics of Sirolimus in Rats
Joint Authors
Zhang, Feng
Sun, Liang
Zhai, Jianxiu
Xia, Tianyi
Jiang, Wei
Li, Mingming
Gao, Shouhong
Tao, Xia
Chen, Wansheng
Chai, Yifeng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
To evaluate the effect of Tanreqing injection on the pharmacokinetics of sirolimus in rats, a high performance liquid chromatography tandem mass spectrometry method was developed for sirolimus assay in whole blood.
Calibration curve of sirolimus was acquired over a concentration ranging from 2.5 to 100 ng/mL with r2= 0.9955.
The matrix effects and extraction recoveries of sirolimus ranged from 144% to 152% and from 80% to 96%, respectively.
The inter- and intraday relative standard deviations were both <10%.
The stability investigation showed that the blood samples were stable for 30-day-storage at -20°C, for 8 h storage at room temperature, for 24 h storage in the auto-sampler at 4°C, and for three freeze-thaw cycle process.
The pharmacokinetic results demonstrated that the Cmax, AUC, and AUMC of sirolimus in rats (7.5 mg/kg, i.g.) were increased after beincoadministration with Tanreqing Injection at 2.5, 5.0, and 7.5 mL/kg (i.v.), respectively, or at 5 min, 2 h, and 4 h (5.0 mL/kg, i.v.) after SRL dosing, respectively.
For the first time, the results proved the herb-drug interaction between Tanreqing Injection and sirolimus and accordingly suggested avoiding concurrent reception of those two drugs for patients.
American Psychological Association (APA)
Zhang, Feng& Sun, Liang& Zhai, Jianxiu& Xia, Tianyi& Jiang, Wei& Li, Mingming…[et al.]. 2019. The Effect of Tanreqing Injection on the Pharmacokinetics of Sirolimus in Rats. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1123544
Modern Language Association (MLA)
Zhang, Feng…[et al.]. The Effect of Tanreqing Injection on the Pharmacokinetics of Sirolimus in Rats. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1123544
American Medical Association (AMA)
Zhang, Feng& Sun, Liang& Zhai, Jianxiu& Xia, Tianyi& Jiang, Wei& Li, Mingming…[et al.]. The Effect of Tanreqing Injection on the Pharmacokinetics of Sirolimus in Rats. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1123544
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123544