The Effect of Triptolide-Loaded Exosomes on the Proliferation and Apoptosis of Human Ovarian Cancer SKOV3 Cells
Joint Authors
Liu, Huan
Shen, Ming
Zhao, De
Ru, Dan
Duan, Yourong
Ding, Chenhuan
Li, He
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-21
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Triptolide has been proven to possess anticancer efficacy; however, its application in the clinical practice was limited by poor water solubility, hepatotoxicity, and nephrotoxicity.
In this study, a triptolide-loaded exosomes delivery system (TP-Exos) was constructed and its effects on the proliferation and apoptosis of SKOV3 cells in vitro and in vivo were observed.
SKOV3-exosomes (SK-Exos) were collected by ultracentrifugation and ultrafiltration centrifugation.
TP-Exos was constructed by sonication and ultrafiltration centrifugation.
SK-Exos and TP-Exos were characterized by transmission electron microscopy, western blotting, nanoparticle-tracking analysis, and high-performance liquid chromatography.
Cellular uptake of exosomes, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, bromodeoxyuridine (BrdU) cell proliferation assay, and cell apoptosis experiment were used to study the effect of TP-Exos on ovarian cancer in vitro.
Tumor-targeting study of exosomes, monitoring the tumor volume of mice, and TdT-mediated dUTP Nick-End labeling (TUNEL) assay were used to evaluate the effect of TP-Exos on ovarian cancer in vivo.
The toxicity of TP-Exos in vivo was evaluated by liver and kidney function and histopathology of major organs (heart, liver, spleen, lung, kidney, and ovary).
The results revealed that TP-Exos not only have the general characteristics of exosomes but also have high drug encapsulation efficiency.
Besides, PKH26 labeled exosomes (PKH26-Exos) could be uptaken by SKOV3 cells, and Dir labeled exosomes (Dir-Exos) could be enriched to the tumor site of tumor bearing mice.
Furthermore, the cytotoxic and apoptotic effects on SKOV3 cells of TP-Exos were weaker than those of free TP, and tumor cell proliferation inhibition and tumor growth inhibition were stronger than that of free TP.
Moreover, TP-Exos have toxic effect on liver and spleen.
In conclusion, the TP-Exos could be a promising strategy for ovarian cancer, but they need to be further optimized to attenuate the damage to liver and spleen.
American Psychological Association (APA)
Liu, Huan& Shen, Ming& Zhao, De& Ru, Dan& Duan, Yourong& Ding, Chenhuan…[et al.]. 2019. The Effect of Triptolide-Loaded Exosomes on the Proliferation and Apoptosis of Human Ovarian Cancer SKOV3 Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1123953
Modern Language Association (MLA)
Liu, Huan…[et al.]. The Effect of Triptolide-Loaded Exosomes on the Proliferation and Apoptosis of Human Ovarian Cancer SKOV3 Cells. BioMed Research International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1123953
American Medical Association (AMA)
Liu, Huan& Shen, Ming& Zhao, De& Ru, Dan& Duan, Yourong& Ding, Chenhuan…[et al.]. The Effect of Triptolide-Loaded Exosomes on the Proliferation and Apoptosis of Human Ovarian Cancer SKOV3 Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1123953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123953