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Extraction and Characterization of Organ Components of the Malaysian Sea Cucumber Holothuria leucospilota Yielded Bioactives Exhibiting Diverse Properties
Joint Authors
Safinar Ismail, Intan
Ceesay, Abdoulie
Aliyu-Paiko, Mohammed
Nazarudin, Muhammad Farhan
Mohamed Alipiah, Norfarrah
Shamsudin, Mariana Nor
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-15
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The aim of the present study was to extract and characterize bioactive components from separate body organs of Holothuria leucospilota.
Preliminary qualitative assessment of the crude extracts was positive for phenols, terpenoids, carbohydrates, flavonoids, saponins, glycosides, cardiac glycosides, steroids, phlobatannins, and tannins in all body organs evaluated.
Phenolics were the most abundant group of bioactives accounting for approximately 80%.
The extraction solvent mixtures that yielded most compounds evaluated were methanol/acetone (3:1, v:v) and methanol/distilled water (3:1, v:v).
In other analyses, GC-MS data revealed diverse metabolic and biologically active compounds, where those in high concentrations included 2-Pentanone, 4-hydroxy-4-methyl- among the ketones; phenol- 2,4-bis(1,1-dimethylethyl)-, a phenol group; and 2-Chlorooctane, a hydrocarbon.
Among FA and their methyl/ethyl esters, n-hexadecanoic acid, 5,8,11,14-eicosatetraenoic acid ethyl ester (arachidonic acid), and 5,8,11,14,17-eicosapentaenoic acid methyl ester (EPA) were among the most abundant FAMEs accounting for approximately 50% of the subgroups measured.
Data from GC-FID analysis revealed methyl laurate (C12:0), methyl myristate (C14:0), methyl palmitate (C16:0), and methyl stearate (18:0) methyl esters as the most abundant saturated FA, whereas cis-9-oleic methyl ester (C18:1) and methyl linoleate (C18:2) were found as the major monounsaturated FA and PUFA FAMEs, respectively, in the body wall of the species.
Taken together, the extraction and characterization of different categories of metabolically and biologically active compounds in various organ extracts of H.
leucospilota suggest that the species is potentially a rich source of cholesterol-lowering, antioxidant, antimicrobial, and anticancer agents.
These substances are known to benefit human health and assist in disease prevention.
These findings justify the use of sea cucumbers in traditional folklore medication and the current interest and attention focused on the species to mine for bioactives in new drugs research.
American Psychological Association (APA)
Ceesay, Abdoulie& Shamsudin, Mariana Nor& Aliyu-Paiko, Mohammed& Safinar Ismail, Intan& Nazarudin, Muhammad Farhan& Mohamed Alipiah, Norfarrah. 2019. Extraction and Characterization of Organ Components of the Malaysian Sea Cucumber Holothuria leucospilota Yielded Bioactives Exhibiting Diverse Properties. BioMed Research International،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1123993
Modern Language Association (MLA)
Ceesay, Abdoulie…[et al.]. Extraction and Characterization of Organ Components of the Malaysian Sea Cucumber Holothuria leucospilota Yielded Bioactives Exhibiting Diverse Properties. BioMed Research International No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1123993
American Medical Association (AMA)
Ceesay, Abdoulie& Shamsudin, Mariana Nor& Aliyu-Paiko, Mohammed& Safinar Ismail, Intan& Nazarudin, Muhammad Farhan& Mohamed Alipiah, Norfarrah. Extraction and Characterization of Organ Components of the Malaysian Sea Cucumber Holothuria leucospilota Yielded Bioactives Exhibiting Diverse Properties. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1123993
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123993