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Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells
Joint Authors
Li, Min-Hui
Ren, Kai
Su, He
Lv, Li-juan
Yi, Le-tai
Gong, Xue
Dang, Lian-sheng
Zhang, Rui-fen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In previous studies, Gentianella acuta (Michx.) Hulten was reported to contain xanthones, iridoids, terpenoids, and sterols and is mainly used to cure hepatitis, jaundice, fever, headache, and angina pectoris.
In this study, we used bioassay guided fractionation to identify compounds from G.
acuta and investigated their activity against hydrogen peroxide (H2O2)-induced apoptosis of H9c2 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.
The levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and glutamate-cysteine ligase catalytic (GCLC) expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR).
Protein expression was evaluated using western blot.
The results showed that all four compounds had protective effects on H9c2 cells.
The transcription levels of HO-1 and GCLC significantly increased in H9c2 cells pretreated with norswertianolin (1), swetrianolin (2), demethylbellidifolin (3), and bellidifolin (4).
However, compared to the model group, the transcription levels of Nrf2 were not enhanced by pretreatment with compounds 1, 2, and 4.
The protein expression levels of HO-1 and GCLC in H9c2 cells were greater than that in the H2O2-treated group, and the expression of Nrf2 was not significantly changed except by swetrianolin treatment; inhibitors can reverse the protective effect by ZnPP (15 μM), BSO (10 μM), and brusatol (10 μM).
The results indicated that the four compounds isolated from G.
acuta inhibited the oxidative injury induced by H2O2 by activating the Nrf2/ARE pathway in H9c2 cells and provide evidence that G.
acuta may be a potential therapeutic agent for the treatment of cardiovascular diseases.
American Psychological Association (APA)
Ren, Kai& Su, He& Lv, Li-juan& Yi, Le-tai& Gong, Xue& Dang, Lian-sheng…[et al.]. 2019. Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124021
Modern Language Association (MLA)
Ren, Kai…[et al.]. Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1124021
American Medical Association (AMA)
Ren, Kai& Su, He& Lv, Li-juan& Yi, Le-tai& Gong, Xue& Dang, Lian-sheng…[et al.]. Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124021
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124021