pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair
Joint Authors
Zhao, Rong-lan
Zhang, Xu-mei
Jia, Li-na
Song, Wei
Sun, Yan-li
Meng, Xiang-ying
Peng, Xiao-xiang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-21
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The aim of the present study was to investigate the effects of phosphorylatable nucleus localization signal linked nucleic kinase substrate short peptide ( N N S p )-conjugated chitosan ( N N S p -CS) mediated miR-140 and IGF-1 in both rabbit chondrocytes and cartilage defects model.
N N S p -CS was combined with pBudCE4.1-IGF-1, pBudCE4.1-miR-140, and negative control pBudCE4.1 to form pDNA/ N N S p -CS complexes.
Then these complexes were transfected into chondrocytes or injected intra-articularly into the knee joints.
High levels of IGF-1 and miR-140 expression were detected both in vitro and in vivo.
Compared with pBudCE4.1 group, in vitro, the transgenic groups significantly promoted chondrocyte proliferation, increased glycosaminoglycan (GAG) synthesis, and ACAN, COL2A1, and TIMP-1 levels, and reduced the levels of nitric oxide (NO), MMP-13, and ADAMTS-5.
In vivo, the exogenous genes enhanced COL2A1, ACAN, and TIMP-1 expression in cartilage and reduced cartilage Mankin score and the contents of NO, IL-1β, TNF-α, and GAG contents in synovial fluid of rabbits, MMP-13, ADAMTS-5, COL1A2, and COL10A1 levels in cartilage.
Double gene combination showed better results than single gene.
This study indicate that N N S p -CS is a better gene delivery vehicle in gene therapy for cartilage defects and that miR-140 combination IGF-1 transfection has better biologic effects on cartilage defects.
American Psychological Association (APA)
Zhao, Rong-lan& Zhang, Xu-mei& Jia, Li-na& Song, Wei& Sun, Yan-li& Meng, Xiang-ying…[et al.]. 2019. pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair. BioMed Research International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1124079
Modern Language Association (MLA)
Zhao, Rong-lan…[et al.]. pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair. BioMed Research International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1124079
American Medical Association (AMA)
Zhao, Rong-lan& Zhang, Xu-mei& Jia, Li-na& Song, Wei& Sun, Yan-li& Meng, Xiang-ying…[et al.]. pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1124079
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124079