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Antimicrobial Resistance and Virulence Genes in Enterococcus faecium and Enterococcus faecalis from Humans and Retail Red Meat
Joint Authors
Golob, Majda
Dermota, Urška
Avberšek, Jana
Papić, Bojan
Zdovc, Irena
Kušar, Darja
Pate, Mateja
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The emergence of antimicrobial-resistant and virulent enterococci is a major public health concern.
While enterococci are commonly found in food of animal origin, the knowledge on their zoonotic potential is limited.
The aim of this study was to determine and compare the antimicrobial susceptibility and virulence traits of Enterococcus faecalis and Enterococcus faecium isolates from human clinical specimens and retail red meat in Slovenia.
A total of 242 isolates were investigated: 101 from humans (71 E.
faecalis, 30 E.
faecium) and 141 from fresh beef and pork (120 E.
faecalis, 21 E.
faecium).
The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of seven common virulence genes was investigated using PCR.
In both species, the distribution of several resistance phenotypes and virulence genes was disparate for isolates of different origin.
All isolates were susceptible to daptomycin, linezolid, teicoplanin, and vancomycin.
In both species, the susceptibility to antimicrobials was strongly associated with a food origin and the multidrug resistance, observed in 29.6% of E.
faecalis and 73.3% E.
faecium clinical isolates, with a clinical origin (Fisher’s exact test).
Among meat isolates, in total 66.0% of E.
faecalis and E.
faecium isolates were susceptible to all antimicrobials tested and 32.6% were resistant to either one or two antimicrobials.
In E.
faecalis, several virulence genes were significantly associated with a clinical origin; the most common (31.0%) gene pattern included all the tested genes except hyl.
In meat isolates, the virulence genes were detected in E.
faecalis only and the most common pattern included ace, efaA, and gelE (32.5%), of which gelE showed a statistically significant association with a clinical origin.
These results emphasize the importance of E.
faecalis in red meat as a reservoir of virulence genes involved in its persistence and human infections with reported severe outcomes.
American Psychological Association (APA)
Golob, Majda& Pate, Mateja& Kušar, Darja& Dermota, Urška& Avberšek, Jana& Papić, Bojan…[et al.]. 2019. Antimicrobial Resistance and Virulence Genes in Enterococcus faecium and Enterococcus faecalis from Humans and Retail Red Meat. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1124104
Modern Language Association (MLA)
Golob, Majda…[et al.]. Antimicrobial Resistance and Virulence Genes in Enterococcus faecium and Enterococcus faecalis from Humans and Retail Red Meat. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1124104
American Medical Association (AMA)
Golob, Majda& Pate, Mateja& Kušar, Darja& Dermota, Urška& Avberšek, Jana& Papić, Bojan…[et al.]. Antimicrobial Resistance and Virulence Genes in Enterococcus faecium and Enterococcus faecalis from Humans and Retail Red Meat. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1124104
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124104