Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells
Joint Authors
Lin, Hongsheng
Liu, Qiuling
Lin, Zhen
Liu, Yi
Du, Jiang
Wang, Jing
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Delivery of osteogenesis-promoting microRNAs (miRNAs) is a promising approach to enhance bone regeneration.
In this study, we generated nanocomplexes comprising the novel cell-penetrating peptide R9-LK15 and miR-29b and investigated their effects on osteogenic differentiation of bone mesenchymal stem cells (BMSCs).
R9-LK15/miR-29b nanocomplexes were prepared and characterized.
The transfection efficiency, cell viability, and osteogenic differentiation were investigated.
The results showed that R9-LK15 maintained the stability of miR-29b in serum for up to 24 h.
Moreover, R9-LK15 efficiently delivered miR-29b into BMSCs; the transfection efficiency was ~10-fold higher than that achieved using Lipofectamine 2000.
The Cell Counting Kit-8 assay showed that R9-LK15 and R9-LK15/miR-29b nanocomplexes had negligible cytotoxic effects on BMSCs.
Delivery of R9-LK15/miR-29b nanocomplexes promoted osteogenic differentiation of BMSCs and extracellular matrix mineralization by upregulating alkaline phosphatase expression and downregulating histone deacetylase-4 expression.
In general, we developed a novel miRNA delivery system that has a high transfection efficiency and promotes osteogenic differentiation.
American Psychological Association (APA)
Liu, Qiuling& Lin, Zhen& Liu, Yi& Du, Jiang& Lin, Hongsheng& Wang, Jing. 2019. Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1124280
Modern Language Association (MLA)
Liu, Qiuling…[et al.]. Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1124280
American Medical Association (AMA)
Liu, Qiuling& Lin, Zhen& Liu, Yi& Du, Jiang& Lin, Hongsheng& Wang, Jing. Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1124280
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124280
