Tetrahydroxystilbene Glucoside Effectively Prevents Apoptosis Induced Hair Loss

Abstract EN

The effect of Polygonum multiflorum against hair loss has been widely recognized.

2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is the main component of Polygonum multiflorum; however, its role in hair regeneration has not been established.

To evaluate the hair growth-promoting activity of TSG, depilated C57BL/6J mice were topically treated with normal saline, TSG, Pifithrin-α, Minoxidil for 2 weeks.

In this study, we identified that p53, Caspase-3, Active Caspase-3, and Caspase-9 were obviously upregulated in the skin of human and mice with hair loss by western blot analysis.

Depilated mice treated with TSG showed markedly hair regrowth.

TUNEL+ cells were also reduced in mice with TSG.

These changes were accompanied with inhibition of Fas, p53, Bax, Active Caspase-3, and Procaspase-9 activities.

These results demonstrated that TSG exerts great hair regrowth effect on hair loss, which was probably mediated by inhibition of p53, Fas, and Bax induced apoptosis.