Analysis of Transcription Factor-Related Regulatory Networks Based on Bioinformatics Analysis and Validation in Hepatocellular Carcinoma
Joint Authors
Du, Zhenwu
Wang, Qingyu
Liu, Shui
Yao, Xiaoxiao
Zhang, Dan
Sheng, Jiyao
Wen, Xin
Chen, Gaoyang
Li, Zhaoyan
Zhang, Xuewen
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-29
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Hepatocellular carcinoma (HCC) accounts for a significant proportion of liver cancer, which has become the second most common cause of cancer-related mortality worldwide.
To investigate the potential mechanisms of invasion and progression of HCC, bioinformatics analysis and validation by qRT-PCR were performed.
We found 237 differentially expressed genes (DEGs) including EGR1, FOS, and FOSB, which were three cancer-related transcription factors.
Subsequently, we constructed TF-gene network and miRNA-TF-mRNA network based on data obtained from mRNA and miRNA expression profiles for analysis of HCC.
We found that 42 key genes from the TF-gene network including EGR1, FOS, and FOSB were most enriched in the p53 signaling pathway.
The qRT-PCR data confirmed that mRNA levels of EGR1, FOS, and FOSB all were decreased in HCC tissues.
In addition, we confirmed that the mRNA levels of CCNB1, CCNB2, and CHEK1, three key markers of the p53 signaling pathway, were all increased in HCC tissues by bioinformatics analysis and qRT-PCR validation.
Therefore, we speculated that miR-181a-5p, which was upregulated in HCC tissues, could regulate FOS and EGR1 to promote the invasion and progression of HCC by p53 signaling pathway.
Overall, the study provides support for the possible mechanisms of progression in HCC.
American Psychological Association (APA)
Liu, Shui& Yao, Xiaoxiao& Zhang, Dan& Sheng, Jiyao& Wen, Xin& Wang, Qingyu…[et al.]. 2018. Analysis of Transcription Factor-Related Regulatory Networks Based on Bioinformatics Analysis and Validation in Hepatocellular Carcinoma. BioMed Research International،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1124312
Modern Language Association (MLA)
Liu, Shui…[et al.]. Analysis of Transcription Factor-Related Regulatory Networks Based on Bioinformatics Analysis and Validation in Hepatocellular Carcinoma. BioMed Research International No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1124312
American Medical Association (AMA)
Liu, Shui& Yao, Xiaoxiao& Zhang, Dan& Sheng, Jiyao& Wen, Xin& Wang, Qingyu…[et al.]. Analysis of Transcription Factor-Related Regulatory Networks Based on Bioinformatics Analysis and Validation in Hepatocellular Carcinoma. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1124312
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124312