Protein Arginine Methyltransferase 2 Inhibits Angiotensin II-Induced Proliferation and Inflammation in Vascular Smooth Muscle Cells
Joint Authors
Zeng, Si-yu
Luo, Jing-fei
Quan, Hai-yan
Xiao, Yun-bin
Liu, Yu-huan
Lu, Hui-qin
Qin, Xu-ping
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objectives.
Protein arginine methyltransferase 2 (PRMT2) protects against vascular injury-induced intimal hyperplasia; however, little is known about the role of PRMT2 in angiotensin II (Ang II)-induced VSMCs proliferation and inflammation.
This research aims to determine whether PRMT2 inhibits Ang II-induced proliferation and inflammation of vascular smooth muscle cells (VSMCs).
Materials and Methods.
PRMT2 overexpression was used to elucidate the role of PRMT2 in Ang II-induced VSMCs proliferation and inflammation.
Western blotting and reverse transcriptional PCR were adopted to detect protein and mRNA expression severally.
Cell viability was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and cell cycle distribution by flow cytometry.
Results.
Ang II significantly reduced mRNA and protein levels of PRMT2 in VSMCs in time-dependent and dose-dependent manner.
Results of PRMT2 overexpression indicated that PRMT2 inhibited proliferation of VSMCs stimulated with 100 nmol/L Ang II for 24 hours.
Furthermore, overexpression of PRMT2 reduced Ang II-induced production of proinflammatory cytokines such as interleukin 6 (IL-6) and interleukin 1β (IL-1β) in VSMCs.
Conclusions.
These findings suggest that PRMT2 alleviates Ang II-induced VSMCs proliferation and inflammation, providing a new mechanism about how Ang II mediated VSMCs proliferation and inflammation.
American Psychological Association (APA)
Zeng, Si-yu& Luo, Jing-fei& Quan, Hai-yan& Xiao, Yun-bin& Liu, Yu-huan& Lu, Hui-qin…[et al.]. 2018. Protein Arginine Methyltransferase 2 Inhibits Angiotensin II-Induced Proliferation and Inflammation in Vascular Smooth Muscle Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1124455
Modern Language Association (MLA)
Zeng, Si-yu…[et al.]. Protein Arginine Methyltransferase 2 Inhibits Angiotensin II-Induced Proliferation and Inflammation in Vascular Smooth Muscle Cells. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1124455
American Medical Association (AMA)
Zeng, Si-yu& Luo, Jing-fei& Quan, Hai-yan& Xiao, Yun-bin& Liu, Yu-huan& Lu, Hui-qin…[et al.]. Protein Arginine Methyltransferase 2 Inhibits Angiotensin II-Induced Proliferation and Inflammation in Vascular Smooth Muscle Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1124455
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124455