Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine
Joint Authors
Hao, Yue
Feng, Yuejin
Li, Jielei
Gu, Xianhong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aim.
To evaluate the role of heat shock protein 70 (HSP70) on the MAPK pathway activation with quercetin treatment and its protection against small intestine impairments of heat stressed rats.
Methods.
Forty-eight male Sprague-Dawley rats aged 6 weeks were randomized to three groups (n=16/group), namely, control (CON), heat stress (HS), and heat stress + quercetin (HQ).
The experiment lasted for 14 days with daily 50 min of heat stress treatment (43°C) for the HS and HQ groups.
Rats of HQ group were intragastrically given 0.5 ml quercetin solution (50 mg/kg body weight) before the heat stress treatment.
Half of the animals were sacrificed on day 7 and the rest on day 14 for tissue sampling.
Intestinal morphology, small intestine morphology and permeability, protein expression of HSP70, phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and caspase-3 activity were examined.
Results.
Heat stress caused morphological damage to the small intestine and increased intestinal permeability.
HSP70 expression and MAPK activity in the small intestine were increased by heat stress.
Inhibition of HSP70 by quercetin did not change intestinal permeability compared with the HS group but aggravated intestinal injury and affected the activation of MAPKs and caspase-3.
Conclusions.
HSP70 may modulate stress-activated signaling and acts in a protective manner via MAPK signaling.
Affecting HSP70 protective mechanisms could be useful for protection against heat stress-induced injury in rat small intestine.
American Psychological Association (APA)
Hao, Yue& Feng, Yuejin& Li, Jielei& Gu, Xianhong. 2018. Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124475
Modern Language Association (MLA)
Hao, Yue…[et al.]. Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1124475
American Medical Association (AMA)
Hao, Yue& Feng, Yuejin& Li, Jielei& Gu, Xianhong. Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124475