Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine

Joint Authors

Hao, Yue
Feng, Yuejin
Li, Jielei
Gu, Xianhong

Source

BioMed Research International

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-07-12

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Aim.

To evaluate the role of heat shock protein 70 (HSP70) on the MAPK pathway activation with quercetin treatment and its protection against small intestine impairments of heat stressed rats.

Methods.

Forty-eight male Sprague-Dawley rats aged 6 weeks were randomized to three groups (n=16/group), namely, control (CON), heat stress (HS), and heat stress + quercetin (HQ).

The experiment lasted for 14 days with daily 50 min of heat stress treatment (43°C) for the HS and HQ groups.

Rats of HQ group were intragastrically given 0.5 ml quercetin solution (50 mg/kg body weight) before the heat stress treatment.

Half of the animals were sacrificed on day 7 and the rest on day 14 for tissue sampling.

Intestinal morphology, small intestine morphology and permeability, protein expression of HSP70, phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and caspase-3 activity were examined.

Results.

Heat stress caused morphological damage to the small intestine and increased intestinal permeability.

HSP70 expression and MAPK activity in the small intestine were increased by heat stress.

Inhibition of HSP70 by quercetin did not change intestinal permeability compared with the HS group but aggravated intestinal injury and affected the activation of MAPKs and caspase-3.

Conclusions.

HSP70 may modulate stress-activated signaling and acts in a protective manner via MAPK signaling.

Affecting HSP70 protective mechanisms could be useful for protection against heat stress-induced injury in rat small intestine.

American Psychological Association (APA)

Hao, Yue& Feng, Yuejin& Li, Jielei& Gu, Xianhong. 2018. Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124475

Modern Language Association (MLA)

Hao, Yue…[et al.]. Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1124475

American Medical Association (AMA)

Hao, Yue& Feng, Yuejin& Li, Jielei& Gu, Xianhong. Role of MAPKs in HSP70’s Protection against Heat Stress-Induced Injury in Rat Small Intestine. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124475

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1124475