Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Joint Authors

Geng, Pei-wu
Zhang, Bin
Liu, Jinlai
Fan, Zhehua
Weng, Ziwei
Chen, Lian-Guo
Lin, Guanyang
Wang, Xianqin

Source

BioMed Research International

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-08-13

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Background and Aims.

The present study aimed to develop a simple and sensitive method for quantitative determination of monocrotaline (MCT) in mouse blood employing ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI/MS/MS) using rhynchophylline as an internal standard.

Methods.

Proteins present in the blood samples were precipitated using acetonitrile.

MCT was separated using a 1.7-μm ethylene bridged hybrid (BEH) C18 column (2.1 mm × 50 mm) with a gradient elution program and a constant flow rate of 0.4 mL/min.

The LC mobile phase consisted of 10 mmol/L ammonium acetate (containing 0.1% formic acid) and acetonitrile.

The total elution time was 4.0 min.

The analytes were detected on a UPLC-ESI mass spectrometer in multiple reaction monitoring (MRM) mode and quantified.

Results.

The new method for the determination of MCT has a satisfactory linear detection range of 1-2000 ng/mL and excellent linearity (r = 0.9971).

The lower limit of quantification (LLOQ) of MCT is 1.0 ng/mL.

Intra- and interassay precisions of MCT were ≤13% with an accuracy from 96.2% to 106.6%.

The average recovery of the new method was >75.0%, and matrix effects were between 89.0% and 94.3%.

Based on the pharmacokinetics data, the bioavailability of MCT in mice was 88.3% after oral administration.

Conclusions.

The results suggest that the newly standardized method for quantitative determination of MCT in whole blood is fast, reliable, specific, sensitive, and suitable for pharmacokinetic studies of MCT after intravenous or intragastric administration.

American Psychological Association (APA)

Chen, Lian-Guo& Zhang, Bin& Liu, Jinlai& Fan, Zhehua& Weng, Ziwei& Geng, Pei-wu…[et al.]. 2018. Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124488

Modern Language Association (MLA)

Chen, Lian-Guo…[et al.]. Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1124488

American Medical Association (AMA)

Chen, Lian-Guo& Zhang, Bin& Liu, Jinlai& Fan, Zhehua& Weng, Ziwei& Geng, Pei-wu…[et al.]. Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124488

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1124488