![](/images/graphics-bg.png)
Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro
Joint Authors
Mazurek, Urszula
Wcisło-Dziadecka, Dominika
Grabarek, Beniamin
Zmarzły, Nikola
Skubis, Aleksandra
Sikora, Bartosz
Kruszniewska-Rajs, Celina
Gola, Joanna
Kucharz, Eugeniusz
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
The aim of this study was to evaluate the influence of adalimumab on expression profile of genes associated with the histaminergic system in Normal Human Dermal Fibroblast (NHDF) cells stimulated with 8.00 μg/ml of adalimumab and the identification of miRNAs regulating these genes’ expression.
Methods.
NHDFs were cultured with or without the presence of adalimumab for 2, 8, and 24 hours.
The expression profile of genes and miRNA were determined with the use of microarray technology.
Results.
Among 22283 ID mRNA, 65 are associated with the histaminergic system.
It can be observed that 15 mRNAs differentiate NHDFs cultures with adalimumab form control.
The analysis of miRNAs showed that, among 1105 ID miRNA, 20 miRNAs are differentiating in cells treated with adalimumab for 2 hours, 9 miRNA after 8 hours, and only 3 miRNAs after 24 hours.
Conclusion.
It was also determined that miRNAs play certain role in the regulation of the expression of genes associated with the histaminergic system.
The results of this study confirmed the possibility of using both genes associated with this system as well as miRNAs regulating their expression, as complementary molecular markers of sensitivity to the adalimumab treatment.
American Psychological Association (APA)
Wcisło-Dziadecka, Dominika& Grabarek, Beniamin& Zmarzły, Nikola& Skubis, Aleksandra& Sikora, Bartosz& Kruszniewska-Rajs, Celina…[et al.]. 2018. Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1124489
Modern Language Association (MLA)
Wcisło-Dziadecka, Dominika…[et al.]. Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1124489
American Medical Association (AMA)
Wcisło-Dziadecka, Dominika& Grabarek, Beniamin& Zmarzły, Nikola& Skubis, Aleksandra& Sikora, Bartosz& Kruszniewska-Rajs, Celina…[et al.]. Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1124489
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124489