Immunological Compatibility of Bone Tissues from Alpha-1,3-galactosyltransferase Knockout Pig for Xenotransplantation
Joint Authors
Hwang, Seongsoo
Kang, Seong Soo
Choi, Seok Hwa
Kim, Se Eun
Kang, Kyung Won
Gu, Suna
Ock, Sun A
Shim, Kyung Mi
Jang, Kwangsik
Lee, Sang-Myeong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We investigated whether the lack of galactosyltransferase (α-Gal) expression in bone tissue is associated with reduced immune response of human peripheral blood mononuclear cells (PBMCs) against pig bone tissue.
When human PBMC obtained from heparinized blood of healthy volunteers was stimulated with bone extracts of pigs with α-1,3-galactosyltransferase knock out (α-Gal KO), the proliferation of human PBMCs and production of proinflammatory cytokines such as TNF-α and IL-1β were significantly reduced compared to those stimulated with bone extracts of wild type (WT) pigs.
In addition, activation of CD4+ helper T cells and production of IL-2, IFN-γ, and IL-17 were reduced upon stimulation with bone tissue extracts from α-Gal KO pigs.
This is possibly due to the lowered activities of the NF-κB, p38, ERK, and JNK signaling pathways.
Our findings can be used to evaluate the compatibility of bone tissues from α-Gal KO pigs with human bone grafting as novel natural biomaterials, thereby increasing the feasibility of future clinical applications.
American Psychological Association (APA)
Kim, Se Eun& Kang, Kyung Won& Gu, Suna& Hwang, Seongsoo& Ock, Sun A& Shim, Kyung Mi…[et al.]. 2018. Immunological Compatibility of Bone Tissues from Alpha-1,3-galactosyltransferase Knockout Pig for Xenotransplantation. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1124496
Modern Language Association (MLA)
Kim, Se Eun…[et al.]. Immunological Compatibility of Bone Tissues from Alpha-1,3-galactosyltransferase Knockout Pig for Xenotransplantation. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1124496
American Medical Association (AMA)
Kim, Se Eun& Kang, Kyung Won& Gu, Suna& Hwang, Seongsoo& Ock, Sun A& Shim, Kyung Mi…[et al.]. Immunological Compatibility of Bone Tissues from Alpha-1,3-galactosyltransferase Knockout Pig for Xenotransplantation. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1124496
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124496