In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)
Joint Authors
Anwar, Mohammad
Achakzai, Jahangir Khan
Kakar, Muhammad Ayub
Kakar, Abdul Manan
Kakar, Shahabuddin
Khan, Javed
Khan, Nazima Yousaf
Khilji, Inayatullah
Tareen, Ajab Khan
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26.
MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA).
GC-MS was used for structural elucidation.
Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line.
Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6.
Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration.
This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity.
In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.
American Psychological Association (APA)
Achakzai, Jahangir Khan& Anwar, Mohammad& Kakar, Muhammad Ayub& Kakar, Abdul Manan& Kakar, Shahabuddin& Khan, Javed…[et al.]. 2019. In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR). BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1124512
Modern Language Association (MLA)
Achakzai, Jahangir Khan…[et al.]. In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR). BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1124512
American Medical Association (AMA)
Achakzai, Jahangir Khan& Anwar, Mohammad& Kakar, Muhammad Ayub& Kakar, Abdul Manan& Kakar, Shahabuddin& Khan, Javed…[et al.]. In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR). BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1124512
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124512