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Induction of Endoplasmic Reticulum Stress Pathway by Green Tea Epigallocatechin-3-Gallate (EGCG) in Colorectal Cancer Cells: Activation of PERKp-eIF2αATF4 and IRE1α
Joint Authors
Ismail, Amin
Norhaizan, Mohd Esa
Md Nesran, Zarith Nameyrra
Shafie, Nurul Husna
Ishak, Amirah Haziyah
Tohid, S. F. Md.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Epigallocatechin-3-gallate (EGCG) is the most abundant bioactive polyphenolic compound among the green tea constituents and has been identified as a potential anticancer agent in colorectal cancer (CRC) studies.
This study was aimed to determine the mechanism of actions of EGCG when targeting the endoplasmic reticulum (ER) stress pathway in CRC.
The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was performed on HT-29 cell line and normal cell line (3T3) to determine the EGCG toxicity.
Next, western blot was done to observe the expression of the related proteins for the ER stress pathway.
The Caspase 3/7 assay was performed to determine the apoptosis induced by EGCG.
The results demonstrated that EGCG treatment was toxic to the HT-29 cell line.
EGCG induced ER stress in HT-29 by upregulating immunoglobulin-binding (BiP), PKR-like endoplasmic reticulum kinase (PERK), phosphorylation of eukaryotic initiation factor 2 alpha subunit (eIF2α), activating transcription 4 (ATF4), and inositol-requiring kinase 1 alpha (IRE1α).
Apoptosis was induced in HT-29 cells after the EGCG treatment, as shown by the Caspase 3/7 activity.
This study indicates that green tea EGCG has the potential to inhibit colorectal cancer cells through the induction of ER stress.
American Psychological Association (APA)
Md Nesran, Zarith Nameyrra& Shafie, Nurul Husna& Ishak, Amirah Haziyah& Norhaizan, Mohd Esa& Ismail, Amin& Tohid, S. F. Md.. 2019. Induction of Endoplasmic Reticulum Stress Pathway by Green Tea Epigallocatechin-3-Gallate (EGCG) in Colorectal Cancer Cells: Activation of PERKp-eIF2αATF4 and IRE1α. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124599
Modern Language Association (MLA)
Md Nesran, Zarith Nameyrra…[et al.]. Induction of Endoplasmic Reticulum Stress Pathway by Green Tea Epigallocatechin-3-Gallate (EGCG) in Colorectal Cancer Cells: Activation of PERKp-eIF2αATF4 and IRE1α. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1124599
American Medical Association (AMA)
Md Nesran, Zarith Nameyrra& Shafie, Nurul Husna& Ishak, Amirah Haziyah& Norhaizan, Mohd Esa& Ismail, Amin& Tohid, S. F. Md.. Induction of Endoplasmic Reticulum Stress Pathway by Green Tea Epigallocatechin-3-Gallate (EGCG) in Colorectal Cancer Cells: Activation of PERKp-eIF2αATF4 and IRE1α. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1124599
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124599