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MicroRNA-9 Enhanced Cisplatin Sensitivity in Nonsmall Cell Lung Cancer Cells by Regulating Eukaryotic Translation Initiation Factor 5A2
Joint Authors
Shao, Guofeng
Zheng, Dawei
Sun, Lebo
Shi, Huoshun
Xu, Guodong
Pan, Qiaoling
Song, Jie
Li, Ni
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We determined the role of microRNA (miR)-9 in regulating cisplatin chemoresistance in nonsmall cell lung cancer (NSCLC) cells.
miR-9 and eukaryotic translation initiation factor 5A2 (eIF5A2) levels were examined by reverse transcription–quantitative PCR.
Cell Counting Kit-8 and the 5-ethynyl-2′-deoxyuridine (EdU) assay were used to determine the effects of miR-9 mimic or inhibitor on NSCLC cell proliferation and viability, respectively.
Bioinformatics was used to analyze the relationship between miR-9 and eIF5A2.
Flow cytometry was used to analyze the percentage of apoptotic cells.
miR-9 mimic enhanced cisplatin sensitivity, while miR-9 inhibitor produced the opposite result.
eIF5A2 was identified as a potential target of miR-9, where miR-9 regulated eIF5A2 expression at mRNA and protein level.
miR-9 mimic decreased the expression of eIF5A2 mRNA and protein, while miR-9 inhibitor increased eIF5A2 expression.
eIF5A2 knockdown resolved the effects of miR-9 mimic or inhibitor on cisplatin sensitivity.
miR-9 may be a potential biomarker for enhancing cisplatin sensitivity by regulating eIF5A2 in NSCLC cells.
American Psychological Association (APA)
Pan, Qiaoling& Sun, Lebo& Zheng, Dawei& Li, Ni& Shi, Huoshun& Song, Jie…[et al.]. 2018. MicroRNA-9 Enhanced Cisplatin Sensitivity in Nonsmall Cell Lung Cancer Cells by Regulating Eukaryotic Translation Initiation Factor 5A2. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1124633
Modern Language Association (MLA)
Pan, Qiaoling…[et al.]. MicroRNA-9 Enhanced Cisplatin Sensitivity in Nonsmall Cell Lung Cancer Cells by Regulating Eukaryotic Translation Initiation Factor 5A2. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1124633
American Medical Association (AMA)
Pan, Qiaoling& Sun, Lebo& Zheng, Dawei& Li, Ni& Shi, Huoshun& Song, Jie…[et al.]. MicroRNA-9 Enhanced Cisplatin Sensitivity in Nonsmall Cell Lung Cancer Cells by Regulating Eukaryotic Translation Initiation Factor 5A2. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1124633
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124633