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Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis
Joint Authors
Chen, Kuang-Den
Nakano, Toshiaki
Hu, Tsung-Hui
Lin, Chih-Che
Goto, Shigeru
Chiu, King-Wah
Chen, Chao-Long
Hsu, Li-Wen
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this study is to elucidate the biogenetic modification of donor and recipient interleukin-28B (IL-28B) genotypes in liver graft biopsies after living donor liver transplantation (LDLT) for chronic hepatitis C virus- (HCV-) related, end-stage liver disease.
Fifty liver graft biopsies were collected from recipients during LDLT treatment for HCV-related, end-stage liver disease.
DNA was extracted from all 50 liver tissues, and the IL-28B single-nucleotide polymorphisms (SNPs) rs8099917 and rs12979860 were studied for allelic discrimination by real-time PCR analysis.
Blood samples were obtained from donors and recipients on postoperative day 0 (POD0), POD7, and POD30.
We randomly selected five liver biopsies and isolated the hepatocytes by laser capture microdissection (LCM) to evaluate genotype modifications resulting from LDLT.
After LDLT, the IL-28B SNP rs8099917 was identified not only in the liver graft biopsies and donors’ sera (TT = 41 : 43; GT = 9 : 5; GG = 0 : 2), but also in liver graft biopsies and recipients’ sera on POD0 (TT = 41 : 44; GT = 9 : 4; GG = 0 : 2), POD7 (TT = 41 : 30; GT = 9 : 18; GG = 0 : 2), and POD30 (TT = 41 : 29; GT = 9 : 19; GG = 0 : 2).
A significant difference was observed between the rs8099917 allele frequencies of liver graft biopsies and recipients’ sera on POD30 (p=0.039).
In addition, a significant difference was also noted between the rs12979860 allele frequencies of liver graft biopsies and donors’ sera (CT = 49 : 39; TT = 1 : 10) (p=0.012) and of liver graft biopsies and recipients’ sera on POD0 (CT = 49 : 39; TT = 1 : 11) (p=0.002), POD7 (CT = 49 : 42; TT = 1 : 8) (p=0.016), and POD30 (CT = 49 : 41; TT = 1 : 9) (p=0.008).
This phenomenon was confirmed by pyrosequencing of hepatocytes isolated by LCM.
Following LDLT, the TT-to-GT IL-28B genotype modification predominated in rs8099917, and the CC-to-CT modification predominated in rs12979860.
In conclusion, these modified phenomena suggested that the selected donor with a predictable and favourable IL-28B genotype will not confer a benefit on the recipient in the living donor liver transplantation setting.
American Psychological Association (APA)
Chiu, King-Wah& Nakano, Toshiaki& Chen, Kuang-Den& Hu, Tsung-Hui& Lin, Chih-Che& Hsu, Li-Wen…[et al.]. 2018. Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1124677
Modern Language Association (MLA)
Chiu, King-Wah…[et al.]. Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1124677
American Medical Association (AMA)
Chiu, King-Wah& Nakano, Toshiaki& Chen, Kuang-Den& Hu, Tsung-Hui& Lin, Chih-Che& Hsu, Li-Wen…[et al.]. Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1124677
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124677