Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae)
Joint Authors
Li, Ke
Xu, Na
Yang, Yu Jing
Zhang, Jin Hui
Yin, Huan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-31
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Mythimna separata is a major agricultural pest with seasonal migrating trait in China.
Formation and regulation mechanism of migration behavior has resulted in a large number of fundamental researches involving quantitative studies of gene expression in this species.
Using appropriate reference gene is critical in RT-qPCR data normalization.
A comprehensive study on the reference genes in M.
separata is lacking.
In this paper, expression stabilities of ten candidate reference genes were evaluated in M.
separata under various biotic and abiotic conditions by employing four different software geNorm, NormFinder, BestKeeper, and the comparative ΔCT method.
The comprehensive stabilities ranking of these genes were suggested by RefFinder.
PKG as a target gene was employed to justify the number of reference genes in four larval tissues and two photoperiod treatments.
Results demonstrate that the first three most stable genes were as follows: EF, CypA, and β-TUB for developmental stages; EF, CypA, and RPL12 for larval tissues; EF, TBP, and β-TUB for adult tissues.
RPL12, β-TUB, and EF for densities; EF, RPL12, and GAPDH for photoperiod treatments; β-TUB, EF, and ATPase for temperature treatments.
Stable reference gene combinations may reduce bias in normalization.
This work provides for the first time a comprehensive list of appropriate reference genes and facilitates future studies on gene function of M.
separata.
American Psychological Association (APA)
Li, Ke& Xu, Na& Yang, Yu Jing& Zhang, Jin Hui& Yin, Huan. 2018. Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae). BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1124679
Modern Language Association (MLA)
Li, Ke…[et al.]. Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae). BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1124679
American Medical Association (AMA)
Li, Ke& Xu, Na& Yang, Yu Jing& Zhang, Jin Hui& Yin, Huan. Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae). BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1124679
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124679