Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria
Joint Authors
Ziadi, Manel
Bouzaiene, Taroub
M’Hir, Sana
Zaafouri, Kaouther
Mokhtar, Ferid
Hamdi, Mokhtar
Boisset-Helbert, Claire
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafiltration (EPS-UF) through a 10 KDa cut-off membrane.
EPS recovery by ultrafiltration was higher than ethanol precipitation for Lactococcus lactis SLT10 and Lactobacillus plantarum C7.
However, it was similar with both methods for Leuconostoc mesenteroides B3.
The monomer composition of the EPS fractions revealed differences in structures and molar ratios between the two studied methods.
EPS isolated from Lactococcus lactis SLT10 are composed of glucose and mannose for EPS-ETOH against glucose, mannose, and rhamnose for EPS-UF.
EPS extracted from Lactobacillus plantarum C7 and Leuconostoc mesenteroides B3 showed similar composition (glucose and mannose) but different molar ratios.
The molecular weights of the different EPS fractions ranged from 11.6±1.83 to 62.4±2.94 kDa.
Molecular weights of EPS-ETOH fractions were higher than those of EPS-UF fractions.
Fourier transform infrared (FTIR) analysis revealed a similarity in the distribution of the functional groups (O-H, C-H, C=O, -COO, and C-O-C) between the EPS isolated from the three strains.
American Psychological Association (APA)
Ziadi, Manel& Bouzaiene, Taroub& M’Hir, Sana& Zaafouri, Kaouther& Mokhtar, Ferid& Hamdi, Mokhtar…[et al.]. 2018. Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1124741
Modern Language Association (MLA)
Ziadi, Manel…[et al.]. Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1124741
American Medical Association (AMA)
Ziadi, Manel& Bouzaiene, Taroub& M’Hir, Sana& Zaafouri, Kaouther& Mokhtar, Ferid& Hamdi, Mokhtar…[et al.]. Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1124741
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124741