Upregulated Na+H+-Exchange Protects Human Colon Cancer Tissue against Intracellular Acidification
Joint Authors
Voss, Ninna C. S.
Kold-Petersen, Henrik
Henningsen, Mikkel B.
Homilius, Casper
Boedtkjer, Ebbe
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Increased metabolism accelerates local acid production in cancer tissue.
The mechanisms eliminating acidic waste products from human colon cancer tissue represent promising therapeutic targets for pharmacological manipulation in order to improve prognosis for the increasing number of patients with colon cancer.
We sampled biopsies of human colonic adenocarcinomas and matched normal colon tissue from patients undergoing colon cancer surgery.
We measured steady-state intracellular pH and rates of net acid extrusion in freshly isolated human colonic crypts based on fluorescence microscopy.
Net acid extrusion was almost entirely (>95%) Na+-dependent.
The capacity for net acid extrusion was increased and steady-state intracellular pH elevated around 0.5 in crypts from colon cancer tissue compared with normal colon tissue irrespective of whether they were investigated in the presence or absence of CO2/HCO3–.
The accelerated net acid extrusion from the human colon cancer tissue was sensitive to the Na+/H+-exchange inhibitor cariporide.
We conclude that enhanced net acid extrusion via Na+/H+-exchange elevates intracellular pH in human colon cancer tissue.
American Psychological Association (APA)
Voss, Ninna C. S.& Kold-Petersen, Henrik& Henningsen, Mikkel B.& Homilius, Casper& Boedtkjer, Ebbe. 2019. Upregulated Na+H+-Exchange Protects Human Colon Cancer Tissue against Intracellular Acidification. BioMed Research International،Vol. 2019, no. 2019, pp.1-5.
https://search.emarefa.net/detail/BIM-1124778
Modern Language Association (MLA)
Voss, Ninna C. S.…[et al.]. Upregulated Na+H+-Exchange Protects Human Colon Cancer Tissue against Intracellular Acidification. BioMed Research International No. 2019 (2019), pp.1-5.
https://search.emarefa.net/detail/BIM-1124778
American Medical Association (AMA)
Voss, Ninna C. S.& Kold-Petersen, Henrik& Henningsen, Mikkel B.& Homilius, Casper& Boedtkjer, Ebbe. Upregulated Na+H+-Exchange Protects Human Colon Cancer Tissue against Intracellular Acidification. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-5.
https://search.emarefa.net/detail/BIM-1124778
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124778