Biotransformation of Geniposide into Genipin by Immobilized Trichoderma reesei and Conformational Study of Genipin
Joint Authors
Yang, Yi-Shun
Zhang, Tong
Ding, Yue
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Trichoderma reesei QM9414, Trichoderma viride 3.316, Aspergillus niger M85, and Aspergillus niger M92 were screened for hydrolyzing geniposide into genipin.
T.
reesei was selected according to the β-glucosidase activity of the fermentation broths using geniposide as a substrate.
T.
reesei was immobilized by embedding method using sodium alginate as the carrier.
Geniposide was hydrolyzed by immobilized T.
reesei at 28°C (200 rpm) for 34 h, and the yield of genipin was 89%.
The product was purified and identified by UV, IR, EIMS, and 1H-NMR.
Since there were two sets of signals in 1H-NMR spectra, a series of experiments were performed and verified that the existence of two conformations was the main reason.
Generally, biotransformation of geniposide into genipin by immobilized T.
reesei provides a promising solution to the genipin production.
American Psychological Association (APA)
Yang, Yi-Shun& Ding, Yue& Zhang, Tong. 2018. Biotransformation of Geniposide into Genipin by Immobilized Trichoderma reesei and Conformational Study of Genipin. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124879
Modern Language Association (MLA)
Yang, Yi-Shun…[et al.]. Biotransformation of Geniposide into Genipin by Immobilized Trichoderma reesei and Conformational Study of Genipin. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1124879
American Medical Association (AMA)
Yang, Yi-Shun& Ding, Yue& Zhang, Tong. Biotransformation of Geniposide into Genipin by Immobilized Trichoderma reesei and Conformational Study of Genipin. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1124879
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124879