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Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance ProteinABCG2
Joint Authors
Verenich, Svetlana
Gerk, Phillip M.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Oxidative stress may arise from a variety of pathologies and results in the formation of toxic and reactive chemical species.
Extensive research has been done to establish mechanisms of formation and cytotoxic effects of a number of different products of oxidation stress including peroxynitrite (PN) and 4-hydroxynonenal (4HNE).
However, relatively few studies have investigated their effects on ATP-binding cassette (ABC) transporters.
The objective of this investigation was to determine the effects of PN and 4HNE on BCRP/ABCG2.
To eliminate the effect of metabolic enzymes, the experiments were carried out with inside-out Sf9 membrane vesicles overexpressing BCRP/ABCG2 using riboflavin as a substrate.
The experiments revealed that PN produced IC50 of about 31.2 ± 2.7 μM, based upon initial concentrations.
The IC50 for 4HNE was estimated to be 92 ± 1.4 μM.
Preincubation of membrane vesicles with either PN or 4HNE caused the maximal rate of transport (Vmax) to drop drastically, up to 19 times, with no or much smaller effect on Km.
Thus, PN and 4NE can inhibit BCRP transport activity.
American Psychological Association (APA)
Verenich, Svetlana& Gerk, Phillip M.. 2019. Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance ProteinABCG2. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1124928
Modern Language Association (MLA)
Verenich, Svetlana& Gerk, Phillip M.. Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance ProteinABCG2. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1124928
American Medical Association (AMA)
Verenich, Svetlana& Gerk, Phillip M.. Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance ProteinABCG2. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1124928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124928