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Second Blood Meal by Female Lutzomyia longipalpis: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and Leishmania Infection
Joint Authors
Genta, Fernando A.
Moraes, C. S.
Aguiar-Martins, K.
Costa, S. G.
Bates, P. A.
Dillon, R. J.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Lutzomyia longipalpis is the main vector of visceral leishmaniasis (VL) in America.
Physiological and molecular mechanisms of Leishmania infection in sand flies have been studied during the first gonotrophic cycle.
There are few studies about these interactions during the second gonotrophic cycle mainly because of the difficulties maintaining sand flies through sequential feeds.
Here we standardized conditions to perform the second blood feed efficiently, and our results show that oviposition is an essential factor for the success of multiple feeds.
We evaluated the impact of the second blood meal on longevity, protein digestion, trypsin activity, and Leishmania mexicana development within L.
longipalpis gut.
Mortality of blood-fed females increases after second blood meal as compared to sugar-fed females.
Trypsin activity was lower during the second gonotrophic cycle.
However, no difference in protein intake was observed between blood meals.
There was no difference in the population size of Leishmania in the gut after both blood meals.
In this work, we presented an optimized protocol for obtaining sufficient numbers of sand fly females fed on a second blood meal, and we described some physiological and parasitological aspects of the second gonotrophic cycle which might influence the vectorial competence of sand flies.
American Psychological Association (APA)
Moraes, C. S.& Aguiar-Martins, K.& Costa, S. G.& Bates, P. A.& Dillon, R. J.& Genta, Fernando A.. 2018. Second Blood Meal by Female Lutzomyia longipalpis: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and Leishmania Infection. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1125076
Modern Language Association (MLA)
Moraes, C. S.…[et al.]. Second Blood Meal by Female Lutzomyia longipalpis: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and Leishmania Infection. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1125076
American Medical Association (AMA)
Moraes, C. S.& Aguiar-Martins, K.& Costa, S. G.& Bates, P. A.& Dillon, R. J.& Genta, Fernando A.. Second Blood Meal by Female Lutzomyia longipalpis: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and Leishmania Infection. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1125076
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125076