IL-13 Contributes to Drug Resistance of NKT-Cell Lymphoma Cells by Regulating ABCC4
Joint Authors
Ni, Mingli
Qin, Beibei
Xie, Ling
Zhang, Xudong
Yang, Jiezhi
Lv, Hongqiong
Yang, Mingyue
Zhang, Mingzhi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Extranodal natural killer/T (NK/T) cell lymphoma, nasal type (ENKTL), represents a rare subtype of T-cell lymphomas with aggressive clinical behavior and is relatively resistant to chemotherapy.
However, there is relatively poor understanding of molecular pathogenesis of multidrug resistance in ENKTL.
Here, we aimed to explore the biological roles and potential mechanism of IL-13 and ABCC4 in multidrug resistance of NK/T-cell lymphoma.
Methods.
ELISA analysis was used to determine the level of serum IL-13 and immunohistochemical analysis was applied to detect the ABCC4 expression level in patients with human NK/T-cell lymphoma.
Western blot assay was employed to measure the expression of ABCC4 in cells.
Lenti-sh-ABCC4 viruses were constructed to knock down ABCC4 in YTS cells.
CCK-8 assay and flow cytometric analysis were performed to detect the effects of IL-13 and ABCC4 on cell proliferation and apoptosis.
CCK-8 assay was conducted to detect the effect of IL-13 and ABCC4 on cell sensitivity to adriamycin (ADM) in YTS cells.
Results.
Levels of serum IL-13 and ABCC4 expression were observed to be upregulated in patients with human NK/T-cell lymphoma.
Moreover, ABCC4 protein expression was also increased in NK/T-cell lymphoma YTS cells compared to the normal NK cells.
Interestingly, IL-13 promoted ABCC4 expression in YTS cells.
IL-13 promoted proliferation and suppressed apoptosis of YTS cells and reversed the effects of ABCC4 knockdown on promotive proliferation and inhibitory apoptosis.
In addition, IL-13 enhanced YTS cell chemotherapy resistance to ADM by promoting ABCC4 expression.
Conclusion.
Our findings concluded that IL-13 inhibited chemotherapy sensitivity of NK/T-cell lymphoma cells by regulating ABCC4, disrupting which may effectively improve the therapy protocols against resistant NK/T-cell lymphoma.
American Psychological Association (APA)
Ni, Mingli& Qin, Beibei& Xie, Ling& Zhang, Xudong& Yang, Jiezhi& Lv, Hongqiong…[et al.]. 2018. IL-13 Contributes to Drug Resistance of NKT-Cell Lymphoma Cells by Regulating ABCC4. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1125143
Modern Language Association (MLA)
Ni, Mingli…[et al.]. IL-13 Contributes to Drug Resistance of NKT-Cell Lymphoma Cells by Regulating ABCC4. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1125143
American Medical Association (AMA)
Ni, Mingli& Qin, Beibei& Xie, Ling& Zhang, Xudong& Yang, Jiezhi& Lv, Hongqiong…[et al.]. IL-13 Contributes to Drug Resistance of NKT-Cell Lymphoma Cells by Regulating ABCC4. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1125143
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125143