Rapid Enkephalin Delivery Using Exosomes to Promote Neurons Recovery in Ischemic Stroke by Inhibiting Neuronal p53Caspase-3
Joint Authors
Liu, Yang
Fu, Naisheng
Su, Jiangli
Wang, Xiumei
Li, Xiaohua
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
No pharmacological treatment is currently available to protect brain from neuronal damage after ischemic stroke.
Recent studies found that enkephalin may play an important role in neuron regeneration.
We assembled a homogeneous size vesicle constituted by transferrin, exosomes, and enkephalin.
Immunofluorescence assay showed that transferrin was combined with the exosomes and enkephalin was packaged into the vesicle; thus this complex was called tar-exo-enkephalin.
In vitro studies were performed using rat primary hippocampal neurons and the results showed that enkephalin decreased p53 and caspase-3 levels to 47.6% and 67.2%, respectively, compared to neurons treated with glutamate, thus inhibiting neuron apoptosis caused by glutamate.
An in vivo experiment in rats was also carried out using a transient middle cerebral artery occlusion (tMCAO)/reperfusion model and tar-exo-enkephalin treatment was performed after tMCAO.
The results showed that tar-exo-enkephalin crossed the blood brain barrier (BBB) and decreased the levels of LDH, p53, caspase-3, and NO by 41.9, 52.6, 45.5, and 57.9% compared to the tMCAO rats, respectively.
In addition, tar-exo-enkephalin improved brain neuron density and neurological score after tMCAO.
These findings suggest that the use of exogenous enkephalin might promote neurological recovery after stroke.
American Psychological Association (APA)
Liu, Yang& Fu, Naisheng& Su, Jiangli& Wang, Xiumei& Li, Xiaohua. 2019. Rapid Enkephalin Delivery Using Exosomes to Promote Neurons Recovery in Ischemic Stroke by Inhibiting Neuronal p53Caspase-3. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125231
Modern Language Association (MLA)
Liu, Yang…[et al.]. Rapid Enkephalin Delivery Using Exosomes to Promote Neurons Recovery in Ischemic Stroke by Inhibiting Neuronal p53Caspase-3. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1125231
American Medical Association (AMA)
Liu, Yang& Fu, Naisheng& Su, Jiangli& Wang, Xiumei& Li, Xiaohua. Rapid Enkephalin Delivery Using Exosomes to Promote Neurons Recovery in Ischemic Stroke by Inhibiting Neuronal p53Caspase-3. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125231
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125231