MiR-221 Promotes Hepatocellular Carcinoma Cells Migration via Targeting PHF2
Joint Authors
Fu, Yi
Liu, Mingyan
Li, Fengxia
Qian, Li
Zhang, Ping
Lv, Fengwei
Cheng, Wenting
Hou, Ruixing
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
MicroRNAs (MiRNAs), which regulate the gene expression leading to translational inhibition or mRNA degradation, are involved in carcinogenesis and tumor progression.
Previous studies have demonstrated that miR-221 was one of the most consistent overexpressed miRNAs in several types of cancer.
However, the role of miR-221 in human liver cancer progression is not yet fully elucidated.
Levels of miR-221 and plant homeodomain finger 2 (PHF2) expressions in human hepatocellular carcinoma (HCC) tissues and cell lines were detected using western blotting and quantitative real-time PCR (qRT-PCR).
Cell migration was studied using the transwell assays.
A dual-luciferase reporter system was used to validate the target gene of miR-221.
The results indicated that miR-221 promoted HCC cell migration.
By performing subsequent systematic bioinformatic analyses, we found PHF2 was the target gene of miR-221 and the direct binding relationship was further validated by dual-luciferase reporter assay.
In addition, lower expression of PHF2 promoted HCC cell migration and linked to worse overall survival in HCC patients.
Finally, the negative correlation between miR-221 and PHF2 expression levels in HCC specimens was further confirmed.
Taken together, our findings implied that miR-221 could be a potential candidate for the therapeutics of HCC metastasis.
American Psychological Association (APA)
Fu, Yi& Liu, Mingyan& Li, Fengxia& Qian, Li& Zhang, Ping& Lv, Fengwei…[et al.]. 2019. MiR-221 Promotes Hepatocellular Carcinoma Cells Migration via Targeting PHF2. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125317
Modern Language Association (MLA)
Fu, Yi…[et al.]. MiR-221 Promotes Hepatocellular Carcinoma Cells Migration via Targeting PHF2. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1125317
American Medical Association (AMA)
Fu, Yi& Liu, Mingyan& Li, Fengxia& Qian, Li& Zhang, Ping& Lv, Fengwei…[et al.]. MiR-221 Promotes Hepatocellular Carcinoma Cells Migration via Targeting PHF2. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125317
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125317