Gene Expression Profiling Confirms the Dosage-Dependent Additive Neuroprotective Effects of Jasminoidin in a Mouse Model of Ischemia-Reperfusion Injury

Joint Authors

Wang, Pengqian
Zhang, Yingying
Liu, Jun
Yu, Yanan
Li, Bing
Wang, Zhong
Li, Haixia
Wang, Jingtao

Source

BioMed Research International

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-05-16

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Medicine

Abstract EN

Recent evidence demonstrates that a double dose of Jasminoidin (2·JA) is more effective than Jasminoidin (JA) in cerebral ischemia therapy, but its dosage-effect mechanisms are unclear.

In this study, the software GeneGo MetaCore was used to perform pathway analysis of the differentially expressed genes obtained in microarrays of mice belonging to four groups (Sham, Vehicle, JA, and 2·JA), aiming to elucidate differences in JA and 2·JA’s dose-dependent pharmacological mechanism from a system’s perspective.

The top 10 enriched pathways in the 2·JA condition were mainly involved in neuroprotection (70% of the pathways), apoptosis and survival (40%), and anti-inflammation (20%), while JA induced pathways were mainly involved in apoptosis and survival (60%), anti-inflammation (20%), and lipid metabolism (20%).

Regarding shared pathways and processes, 3, 1, and 3 pathways overlapped between the Vehicle and JA, Vehicle and 2·JA, and JA and 2·JA conditions, respectively; for the top ten overlapped processes these numbers were 3, 0, and 4, respectively.

The common pathways and processes in the 2·JA condition included differentially expressed genes significantly different from those in JA.

Seven representative pathways were only activated by 2·JA, such as Gamma-Secretase regulation of neuronal cell development.

Process network comparison indicated that significant nodes, such as alpha-MSH, ACTH, PKR1, and WNT, were involved in the pharmacological mechanism of 2·JA.

Function distribution was different between JA and 2·JA groups, indicating a dosage additive mechanism in cerebral ischemia treatment.

Such systemic approach based on whole-genome multiple pathways and networks may provide an effective and alternative approach to identify alterations underlining dosage-dependent therapeutic benefits of pharmacological compounds on complex disease processes.

American Psychological Association (APA)

Li, Haixia& Wang, Jingtao& Wang, Pengqian& Zhang, Yingying& Liu, Jun& Yu, Yanan…[et al.]. 2018. Gene Expression Profiling Confirms the Dosage-Dependent Additive Neuroprotective Effects of Jasminoidin in a Mouse Model of Ischemia-Reperfusion Injury. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125328

Modern Language Association (MLA)

Li, Haixia…[et al.]. Gene Expression Profiling Confirms the Dosage-Dependent Additive Neuroprotective Effects of Jasminoidin in a Mouse Model of Ischemia-Reperfusion Injury. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1125328

American Medical Association (AMA)

Li, Haixia& Wang, Jingtao& Wang, Pengqian& Zhang, Yingying& Liu, Jun& Yu, Yanan…[et al.]. Gene Expression Profiling Confirms the Dosage-Dependent Additive Neuroprotective Effects of Jasminoidin in a Mouse Model of Ischemia-Reperfusion Injury. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125328

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1125328