Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane

Joint Authors

Chen, Wei-Yu
Kraková, Lucia
Wu, Jer-Horng
Pangallo, Domenico
Jeszeová, Lenka
Liu, Bing
Yasui, Hidenari

Source

Archaea

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-12-17

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Biology

Abstract EN

Tetramethylammonium-degrading methanogenic consortia from a complete-mixing suspended sludge (CMSS) and an upflow anaerobic sludge blanket (UASB) reactors were studied using multiple PCR-based molecular techniques and shotgun proteomic approach.

The prokaryotic 16S rRNA genes of the consortia were analyzed by quantitative PCR, high-throughput sequencing, and DGGE-cloning methods.

The results showed that methanogenic archaea were highly predominant in both reactors but differed markedly according to community structure.

Community and proteomic analysis revealed that Methanomethylovorans and Methanosarcina were the major players for the demethylation of methylated substrates and methane formation through the reduction pathway of methyl-S-CoM and possibly, acetyl-CoA synthase/decarbonylase-related pathways.

Unlike high dominance of one Methanomethylovorans population in the CMSS reactor, diverse methylotrophic Methanosarcina species inhabited in syntrophy-like association with hydrogenotrophic Methanobacterium in the granular sludge of UASB reactor.

The overall findings indicated the reactor-dependent community structures of quaternary amines degradation and provided microbial insight for the improved understanding of engineering application.

American Psychological Association (APA)

Chen, Wei-Yu& Kraková, Lucia& Wu, Jer-Horng& Pangallo, Domenico& Jeszeová, Lenka& Liu, Bing…[et al.]. 2017. Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane. Archaea،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1125445

Modern Language Association (MLA)

Chen, Wei-Yu…[et al.]. Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane. Archaea No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1125445

American Medical Association (AMA)

Chen, Wei-Yu& Kraková, Lucia& Wu, Jer-Horng& Pangallo, Domenico& Jeszeová, Lenka& Liu, Bing…[et al.]. Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane. Archaea. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1125445

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1125445