Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes
Joint Authors
Deppenmeier, Uwe
Kröninger, Lena
Gottschling, Jacqueline
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
DNA sequence analysis of the human gut revealed the presence a seventh order of methanogens referred to as Methanomassiliicoccales.
Methanomassiliicoccus luminyensis is the only member of this order that grows in pure culture.
Here, we show that the organism has a doubling time of 1.8 d with methanol + H2 and a growth yield of 2.4 g dry weight/mol CH4.
M.
luminyensis also uses methylamines + H2 (monomethylamine, dimethylamine, and trimethylamine) with doubling times of 2.1–2.3 d.
Similar cell yields were obtained with equimolar concentrations of methanol and methylamines with respect to their methyl group contents.
The transcript levels of genes encoding proteins involved in substrate utilization indicated increased amounts of mRNA from the mtaBC2 gene cluster in methanol-grown cells.
When methylamines were used as substrates, mRNA of the mtb/mtt operon and of the mtmBC1 cluster were found in high abundance.
The transcript level of mtaC2 was almost identical in methanol- and methylamine-grown cells, indicating that genes for methanol utilization were constitutively expressed in high amounts.
The same observation was made with resting cells where methanol always yielded the highest CH4 production rate independently from the growth substrate.
Hence, M.
luminyensis is adapted to habitats that provide methanol + H2 as substrates.
American Psychological Association (APA)
Kröninger, Lena& Gottschling, Jacqueline& Deppenmeier, Uwe. 2017. Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes. Archaea،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1125448
Modern Language Association (MLA)
Kröninger, Lena…[et al.]. Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes. Archaea No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1125448
American Medical Association (AMA)
Kröninger, Lena& Gottschling, Jacqueline& Deppenmeier, Uwe. Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes. Archaea. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1125448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125448