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Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1
Joint Authors
Ho, Thien-Hoang
Huynh, Kim-Hung
Nguyen, Diem Quynh
Park, Hyunjae
Jung, Kyoungho
Sur, Bookyo
Ahn, Yeh-Jin
Cha, Sun-Shin
Kang, Lin-Woo
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Thermococcus onnurineus NA1 is an anaerobic archaeon usually found in a deep-sea hydrothermal vent area, which can use elemental sulfur (S0) as a terminal electron acceptor for energy.
Sulfur, essential to many biomolecules such as sulfur-containing amino acids and cofactors including iron-sulfur cluster, is usually mobilized from cysteine by the pyridoxal 5′-phosphate- (PLP-) dependent enzyme of cysteine desulfurase (CDS).
We determined the crystal structures of CDS from Thermococcus onnurineus NA1 (ToCDS), which include native internal aldimine (NAT), gem-diamine (GD) with alanine, internal aldimine structure with existing alanine (IAA), and internal aldimine with persulfide-bound Cys356 (PSF) structures.
The catalytic intermediate structures showed the dihedral angle rotation of Schiff-base linkage relative to the PLP pyridine ring.
The ToCDS structures were compared with bacterial CDS structures, which will help us to understand the role and catalytic mechanism of ToCDS in the archaeon Thermococcus onnurineus NA1.
American Psychological Association (APA)
Ho, Thien-Hoang& Huynh, Kim-Hung& Nguyen, Diem Quynh& Park, Hyunjae& Jung, Kyoungho& Sur, Bookyo…[et al.]. 2017. Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1. Archaea،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1125457
Modern Language Association (MLA)
Ho, Thien-Hoang…[et al.]. Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1. Archaea No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1125457
American Medical Association (AMA)
Ho, Thien-Hoang& Huynh, Kim-Hung& Nguyen, Diem Quynh& Park, Hyunjae& Jung, Kyoungho& Sur, Bookyo…[et al.]. Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1. Archaea. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1125457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125457