Whole Peptidoglycan Extracts from the Lactobacillus paracasei subsp. paracasei M5 Strain Exert Anticancer Activity In Vitro
Joint Authors
Han, Xue
Zhang, Lanwei
Wang, Shumei
Zhang, Yingchun
Li, Hongbo
Jiao, Yuehua
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The Lactobacillus paracasei subsp.
paracasei M5 strain exerted potential anticancer activity through the cell wall.
In this study, whole peptidoglycan (WPG) was extracted from the Lactobacillus paracasei subsp.
paracasei M5 strain and was evaluated for anticancer effects as well as its properties.
SDS-PAGE analysis confirmed the presence of WPG with dominant bands of approximately 14.4 kDa.
Further analysis revealed that the amino acids present in the WPG consisted of alanine, glycine, glutamic acid, and lysine in a molar ratio of approximately 8 : 5 : 3 : 3.5.
In addition, the cell viability of HT-29 cells with WPG addition was investigated with methyl thiazolyl tetrazolium (MTT) and trypan blue exclusion (TBE) assays, and cell apoptosis was analyzed with a transmission electron microscope, flow cytometry, and semiquantitative RT-PCR.
These results showed that WPG exerted cytotoxic effects on colon cancer HT-29 cells in a dose-dependent manner and upregulated proapoptotic genes, while downregulating antiapoptotic genes.
The gene expression study definitively revealed that WPG induced a mitochondria-mediated apoptotic pathway.
American Psychological Association (APA)
Wang, Shumei& Han, Xue& Zhang, Lanwei& Zhang, Yingchun& Li, Hongbo& Jiao, Yuehua. 2018. Whole Peptidoglycan Extracts from the Lactobacillus paracasei subsp. paracasei M5 Strain Exert Anticancer Activity In Vitro. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125458
Modern Language Association (MLA)
Wang, Shumei…[et al.]. Whole Peptidoglycan Extracts from the Lactobacillus paracasei subsp. paracasei M5 Strain Exert Anticancer Activity In Vitro. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1125458
American Medical Association (AMA)
Wang, Shumei& Han, Xue& Zhang, Lanwei& Zhang, Yingchun& Li, Hongbo& Jiao, Yuehua. Whole Peptidoglycan Extracts from the Lactobacillus paracasei subsp. paracasei M5 Strain Exert Anticancer Activity In Vitro. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125458
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125458